| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
TBTA targets copper ions in CuAAC reactions. As a tetradentate ligand, it coordinates copper(I) in a tripodal geometry, stabilizing the metal ion and preventing its oxidation to copper(II). This stabilization enhances the catalytic efficiency of the CuAAC reaction and reduces side reactions. TBTA is a key component of the “click chemistry” toolbox, enabling the rapid and reliable formation of 1,2,3-triazoles from azides and alkynes. Its mechanism involves coordination to copper(I) and facilitation of the cycloaddition reaction.
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| ln Vitro |
Tris(benzyltriazolylmethyl)amine is a novel ligand that has demonstrated promising results in maintaining Cu+ ions available for chemical reactions while stabilizing Cu+ and Cu2+ ions in the reaction environment, whether with sodium atmosphere or anhydrous conditions[1].
TBTA exhibits in vitro activity as a copper(I)-stabilizing ligand in CuAAC reactions. It enhances the catalytic efficiency and selectivity of the reaction. The compound’s activity is assessed by monitoring the progress of CuAAC reactions, typically by measuring product formation using analytical techniques such as HPLC or mass spectrometry. TBTA has demonstrated promising results in maintaining Cu+ ions available for chemical reactions while stabilizing Cu+ and Cu2+ ions. These in vitro activities confirm its utility as a click chemistry ligand. |
| ln Vivo |
TBTA is not used as a therapeutic agent in vivo. It is primarily used as a chemical reagent in research and industrial applications. Its in vivo effects are not relevant to its primary use as a click chemistry ligand. The compound is intended for research use only.
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| Enzyme Assay |
In vitro assays for TBTA are chemical rather than biological. The compound’s ability to stabilize copper(I) and enhance CuAAC reactions is assessed by monitoring the progress of model cycloaddition reactions. Reaction conversion is measured by analytical techniques such as HPLC, LC-MS, or NMR. These assays confirm the compound’s utility as a click chemistry ligand.
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| Cell Assay |
TBTA is not typically used in cellular assays, as it is a chemical reagent rather than a biological agent. However, its biocompatibility may be assessed in the context of click chemistry applications in living systems. Cytotoxicity assays in mammalian cell lines may be performed to determine the compound’s safety for biological applications.
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| Animal Protocol |
TBTA is not used in animal experiments as a therapeutic agent. Its biocompatibility may be assessed in animal models for click chemistry applications. The compound is administered via injection, and its distribution and effects are assessed. These studies evaluate the compound’s suitability for in vivo click chemistry applications.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for TBTA are not relevant to its use as a chemical reagent. The compound has a molecular weight of 530.63 and is soluble in organic solvents. It is typically stored at room temperature. Further PK studies would be needed if the compound were to be used in biological applications.
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| Toxicity/Toxicokinetics |
TBTA is considered to have low toxicity based on its use as a research compound. However, comprehensive toxicological evaluations have not been extensively published. The compound is intended for research use only and is not approved for human therapeutic use. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
TBTA is a tetradentate ligand used in copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions. It is also known as tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine. TBTA stabilizes copper(I) ions and enhances catalytic efficiency and selectivity. It is a key component of the “click chemistry” toolbox. The compound is available in high purity for research applications. Its versatility makes it a valuable tool in chemical biology and drug discovery.
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| Molecular Formula |
C30H30N10
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|---|---|
| Molecular Weight |
530.6262
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| Exact Mass |
530.265
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| CAS # |
510758-28-8
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| PubChem CID |
11203363
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
762.4±70.0 °C at 760 mmHg
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| Melting Point |
132-143℃
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| Flash Point |
414.9±35.7 °C
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| Vapour Pressure |
0.0±2.6 mmHg at 25°C
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| Index of Refraction |
1.696
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| LogP |
3.18
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
40
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| Complexity |
628
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
WKGZJBVXZWCZQC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C30H30N10/c1-4-10-25(11-5-1)16-38-22-28(31-34-38)19-37(20-29-23-39(35-32-29)17-26-12-6-2-7-13-26)21-30-24-40(36-33-30)18-27-14-8-3-9-15-27/h1-15,22-24H,16-21H2
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| Chemical Name |
1-(1-benzyltriazol-4-yl)-N,N-bis[(1-benzyltriazol-4-yl)methyl]methanamine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~188.46 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (9.42 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 5 mg/mL (9.42 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 5 mg/mL (9.42 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8846 mL | 9.4228 mL | 18.8455 mL | |
| 5 mM | 0.3769 mL | 1.8846 mL | 3.7691 mL | |
| 10 mM | 0.1885 mL | 0.9423 mL | 1.8846 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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