MMP-1; ERK; JNK; p38 MAPK

Trans-Zeatin pretreatment significantly reduces the amount of UVB-induced MMP-1 expression and c-Jun activation in cultured human skin fibroblasts. This effect may be due to the inhibition of the ERK, JNK, and p38 MAPK signaling pathways[1].

In a 96-well plate, HSFs are sown. Cells are cultured in 100 μl of 10% DMEM in each well for the specified amount of time after receiving varying doses of trans-Zeatin or UVB treatment. The cells are then incubated for 4 hours at 37 °C with 10 μl of MTT solution at a concentration of 5 mg/ml in each well. After removing the medium, each well receives 200 μl of DMSO, which is then pipetted up and down repeatedly to dissolve the formazan crystals. A spectrophotometer is used to measure the absorbance at a wavelength of 570 nm on an ELISA plate reader.

Interactions
... Zeatin mitigated cognitive deficits and showed AChE inhibition in scopolamine (Scop)-induced mice following 21 days of zeatin treatment. After administration of Scop for 30 min, each mouse performed Y-maze and step-down latency tasks as a check on immediate against cognitive function. The results showed that zeatin administration attenuated Scop-induced memory damage and decreased AChE activity in the mice...

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Non-Human Toxicity Values
LD50 Rat oral >5 g/kg /from table/ /Cytokinin/
LD50 Rabbit dermal >2 g/kg /from table/ /Cytokinin/

[1]. Int J Mol Med . 2009 Apr;23(4):555-60.

Zeatin is a 6-isopentenylaminopurine. It has a role as a cytokinin.
An aminopurine factor in plant extracts that induces cell division. (Grant & Hackh's Chemical Dict, 5th ed)
trans-Zeatin has been reported in Mercurialis ambigua, Solanum tuberosum, and other organisms with data available.
An aminopurine factor in plant extracts that induces cell division. (Grant and Hackh's Chemical Dict, 5th ed)

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Mechanism of Action
Kinetin stimulated phosphorylation of protein in floated Chinese-cabbage leaf discs, but inhibited protein phosphorylation in nuclei+chloroplast extracts from Chinese-cabbage or tobacco leaves. Kinetin also inhibited protein phosphorylation in isolated tobacco nuclei or nuclei from carrot secondary-phloem tissue. Purified Chinese-cabbage leaf ribosomes exhibited protein kinase activity which was inhibited by kinetin and zeatin. The ribosome-associated kinase responded to kinetin and zeatin differently from that associated with nuclei+chloroplast preparations. Protein phosphorylation in vitro was not affected by adenosine 3':5'-cyclic monophosphate, indol-3-ylacetic acid or gibberellic acid. It was only inhibited by N(9)-unsubstituted purines, among which the known cytokinins were the most effective inhibitors. The results are discussed in relation to possible similarities between the effects of cytokinins in plant tissues and the effects of adenosine 3':5'-cyclic monophosphate in animal tissues. Both compounds appear to modify the activity of protein kinases and both affect many different cellular processes.

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Therapeutic Uses
/EXPL THER/ In this study, our aim was to clarify the ameliorative effects of zeatin, a development hormone in plants. Zeatin mitigated cognitive deficits and showed AChE inhibition in scopolamine (Scop)-induced mice following 21 days of zeatin treatment. After administration of Scop for 30 min, each mouse performed Y-maze and step-down latency tasks as a check on immediate against cognitive function. The results showed that zeatin administration attenuated Scop-induced memory damage and decreased AChE activity in the mice. This suggests that zeatin might be useful for protecting cognitive dysfunction, as well as for reducing the activation of AChE in dementia.

C10H13N5O

219.2431

219.112

C, 54.78; H, 5.98; N, 31.94; O, 7.30

1637-39-4

trans-Zeatin-d5;72963-19-0

449093

White to off-white solid powder

1.4±0.1 g/cm3

395.0±52.0 °C at 760 mmHg

207 °C

192.7±30.7 °C

0.0±2.1 mmHg at 25°C

1.684

-1.02

3

5

4

16

258

0

O([H])C([H])([H])/C(/C([H])([H])[H])=C(\[H])/C([H])([H])N([H])C1C2=C(N=C([H])N=1)N=C([H])N2[H]

UZKQTCBAMSWPJD-FARCUNLSSA-N

InChI=1S/C10H13N5O/c1-7(4-16)2-3-11-9-8-10(13-5-12-8)15-6-14-9/h2,5-6,16H,3-4H2,1H3,(H2,11,12,13,14,15)/b7-2+

(E)-2-methyl-4-(7H-purin-6-ylamino)but-2-en-1-ol

Trans Zeatin; (E)-Zeatin; Zeatine; Zeatin

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

DMSO: 25~43 mg/mL (114.0~196.1 mM)
Ethanol: ~2 mg/mL (~9.1 mM)

Solubility in Formulation 1: ≥ 1.25 mg/mL (5.70 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.

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Solubility in Formulation 2: ≥ 1.25 mg/mL (5.70 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.

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Solubility in Formulation 3: ≥ 1.25 mg/mL (5.70 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.

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 (Please use freshly prepared in vivo formulations for optimal results.)