| Size | Price | |
|---|---|---|
| Other Sizes |
| ln Vitro |
Following treatment with tosyl chloride, Gram-positive bacterial growth was inhibited by 95% to 100%, regardless of dose, with or without serum. E. coli (Gram-negative; with/without serum) was decreased by 94% to 100% at antimicrobial doses of 300 and 400 ppm. At a concentration of 200 ppm, E. coli growth was totally suppressed without serum and 50% inhibited with serum. At 100 and 200 ppm, cell viability remained above 90% under all testing conditions. Exposure to 300 ppm tosyl chloride for 3 minutes can result in cell viability as high as 70%, with longer exposure leading to reduced viability. Serum does not influence cell viability under any conditions [1].
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|---|---|
| ln Vivo |
Rat tissues surrounding the terminal airways of the lungs of both male and female rats showed significant dose-dependent DNA damage and inflammation [2]. Toxic to crayfish, a twenty-four-hour exposure to 50 mg/L of chloramine-T causes significant energy losses that manifest during future physical stress [3]. Through metabolic activation and/or the build-up of reactive metabolites, tosyl chloride may increase the toxicity of numerous xenobiotics. The activity of CYP2E1, CYP1A1/2, CYP2B1/2, CYP3A4, and CYP4A1/2 enzymes were considerably elevated in a dose-dependent manner following treatment with tosyl chloride at 2.50, 5 and 10 mg/kg body weight/day. After receiving 1.25 mg/kg body weight/day of tosyl chloride therapy, this effect was not noticed [4].
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| References |
[1]. Kloth LC, et al. Bactericidal and cytotoxic effects of chloramine-T on wound pathogens and human fibroblasts in vitro. Adv Skin Wound Care. 2007 Jun;20(6):331-45.
[2]. Shim I, et al. Inhalation exposure to chloramine T induces DNA damage and inflammation in lung of Sprague-Dawley rats. J Toxicol Sci. 2013;38(6):937-46. [3]. Kuklina I, et al. Investigation of chloramine-T impact on crayfish Astacus leptodactylus (Esch., 1823) cardiac activity. Environ Sci Pollut Res Int. 2014 Sep;21(17):10262-9. [4]. Martínez MA, et al. Induction of cytochrome P450-dependent mixed function oxidase activities and peroxisome proliferation by chloramine-T in male rat liver. Food Chem Toxicol. 2017 Aug;106(Pt A):86-91 |
| Additional Infomation |
See also: chloramine-t anhydrous (has active moiety).
|
| Molecular Formula |
C7H13CLNNAO5S
|
|---|---|
| Molecular Weight |
281.6896
|
| Exact Mass |
281.01
|
| CAS # |
7080-50-4
|
| PubChem CID |
517414
|
| Appearance |
White to off-white solid powder
|
| Melting Point |
167-170 °C(lit.)
|
| Flash Point |
92 °C
|
| LogP |
3.099
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| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
16
|
| Complexity |
231
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
Cl[N-]S(C1C([H])=C([H])C(C([H])([H])[H])=C([H])C=1[H])(=O)=O.[Na+].O([H])[H].O([H])[H].O([H])[H]
|
| InChi Key |
NZYOAGBNMCVQIV-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C7H7ClNO2S.Na.3H2O/c1-6-2-4-7(5-3-6)12(10,11)9-8;;;;/h2-5H,1H3;;3*1H2/q-1;+1;;;
|
| Chemical Name |
sodium;chloro-(4-methylphenyl)sulfonylazanide;trihydrate
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~355.00 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.88 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.88 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.88 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5500 mL | 17.7500 mL | 35.5000 mL | |
| 5 mM | 0.7100 mL | 3.5500 mL | 7.1000 mL | |
| 10 mM | 0.3550 mL | 1.7750 mL | 3.5500 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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