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| Other Sizes |
| Targets |
TOOS does not have a traditional therapeutic target. It is a chromogenic reagent used in diagnostic assays. Its mechanism of action involves a peroxidase-catalyzed oxidative coupling reaction with hydrogen peroxide to produce a colored product. In the presence of peroxidase, TOOS reacts with hydrogen peroxide to form a quinoneimine dye, which can be measured spectrophotometrically. This reaction is the basis for many enzymatic assays for the determination of various analytes.
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| ln Vitro |
TOOS is not a therapeutic agent with pharmacological in vitro activity. It is a biochemical reagent used in diagnostic and research applications for the detection of hydrogen peroxide and various analytes.
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| ln Vivo |
TOOS is not used as a therapeutic agent in vivo. It is a biochemical reagent used in diagnostic and research applications. No in vivo pharmacological activity data is relevant.
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| Enzyme Assay |
TOOS is not used in enzyme/receptor binding assays as a test compound. It is used as a chromogenic substrate in peroxidase-based assays for the detection of hydrogen peroxide. The assay involves incubating the sample with TOOS, peroxidase, and 4-aminoantipyrine (or similar coupler). The presence of hydrogen peroxide leads to the formation of a colored product, which is measured spectrophotometrically.
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| Cell Assay |
TOOS is not used in cell-based pharmacological assays as a test compound. It is a biochemical reagent used in diagnostic applications and biochemical assays.
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| Animal Protocol |
TOOS is not used in animal models as a therapeutic agent. It is a biochemical reagent.
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| ADME/Pharmacokinetics |
TOOS (CAS 82692-93-1) has a molecular formula of C12H18NNaO4S and a molecular weight of 295.33 g/mol. It is also known as Sodium 3-[Ethyl(m-tolyl)amino]-2-hydroxy-1-propanesulfonate. Appearance: white crystalline powder. Melting point: 249-254°C. Solubility: soluble in water. Storage: 2-8°C (refrigerate). Shelf life: 60 months. Purity: ≥98%.
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| Toxicity/Toxicokinetics |
No toxicity data is publicly available. As a laboratory reagent, TOOS should be handled with standard laboratory safety precautions. It is not intended for human consumption or therapeutic use.
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| References | |
| Additional Infomation |
TOOS is a research-grade biochemical reagent, not a drug. It is used in diagnostic testing and biochemical assays for the enzymatic photometric determination of hydrogen peroxide. It is a Trinder's reagent widely used in clinical chemistry for the determination of various analytes. No clinical trials have been reported.
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| Molecular Formula |
C12H18NO4S-.NA+
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|---|---|
| Molecular Weight |
295.33042
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| Exact Mass |
295.085
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| CAS # |
82692-93-1
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| PubChem CID |
23676159
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| Appearance |
White to off-white solid powder
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| LogP |
1.808
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
19
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| Complexity |
344
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
IRQRBVOQGUPTLG-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C12H19NO4S.Na/c1-3-13(8-12(14)9-18(15,16)17)11-6-4-5-10(2)7-11;/h4-7,12,14H,3,8-9H2,1-2H3,(H,15,16,17);/q;+1/p-1
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| Chemical Name |
sodium;3-(N-ethyl-3-methylanilino)-2-hydroxypropane-1-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~338.60 mM)
DMSO : ≥ 47 mg/mL (~159.14 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.47 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.47 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.47 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3860 mL | 16.9302 mL | 33.8604 mL | |
| 5 mM | 0.6772 mL | 3.3860 mL | 6.7721 mL | |
| 10 mM | 0.3386 mL | 1.6930 mL | 3.3860 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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