| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
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| ln Vitro |
Cyclophilin A protein expression was reduced by 75.9% when TMN 355 (0.5–10 μM) was administered for three to nine hours. After six hours of activation, 1 μM TMN 355 inhibits cyclophilin A [1].
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| Cell Assay |
Western Blot Analysis[1]
Cell Types: THP Cell Line Tested Concentrations: 0.5, 1, 2.5, 5 and 10 μM Incubation Duration: 3, 6 and 9 hrs (hours) Experimental Results: Resulted in 75.9% reduction in cyclophilin A protein expression. |
| References |
[1]. Ramachandran S, et al. Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. Cardiovasc Diabetol. 2016 Nov 3;15(1):152.
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| Molecular Formula |
C21H14CLFN2O2
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|---|---|
| Molecular Weight |
380.8034
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| CAS # |
1186372-20-2
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| SMILES |
ClC1C=CC=C(F)C=1C(NC(NC1C2=C(C=CC=C2)C2C1=CC=CC=2)=O)=O
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| Chemical Name |
2-chloro-N-[(9H-fluoren-9-ylamino)carbonyl]-6-fluoro-benzamide
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| Synonyms |
Compound 3i TMN 355 TMN-355 TMN355
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~31.25 mg/mL (~82.06 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.46 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6261 mL | 13.1303 mL | 26.2605 mL | |
| 5 mM | 0.5252 mL | 2.6261 mL | 5.2521 mL | |
| 10 mM | 0.2626 mL | 1.3130 mL | 2.6261 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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