| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 25mg |
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| Other Sizes |
| Targets |
Temocillin targets penicillin-binding proteins (PBPs) located on the inner membrane of the bacterial cell wall. By binding to and inactivating these PBPs, it inhibits bacterial cell wall synthesis, which is essential for the growth and survival of bacterial cells. Its resistance to β-lactamases makes it effective against many β-lactamase-producing organisms.
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|---|---|
| ln Vitro |
In vitro, temocillin possesses antibacterial activity against a range of Gram-negative bacteria. It is effective against many multidrug-resistant strains. Its in vitro activity is typically assessed by determining the minimum inhibitory concentration (MIC) against a panel of bacterial isolates.
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| ln Vivo |
With no discernible difference between the parental strain and its transconjugant, temocillin dosage regimens of 200 mg/kg every 4 and 6 hours continued to be highly effective in lowering bacterial titres in kidneys against the two strains[2].
In vivo, temocillin is used as an alternative to carbapenems and is indicated for the treatment of septicemia, urinary tract infections, and lower respiratory tract infections. In animal models, temocillin dosage regimens of 200 mg/kg every 4 and 6 hours have been shown to be highly effective in lowering bacterial titers in kidneys. |
| Enzyme Assay |
The in vitro assay for temocillin is a standard antimicrobial susceptibility test. The minimum inhibitory concentration (MIC) is determined by broth microdilution or disk diffusion methods according to CLSI or EUCAST guidelines. The compound's activity is evaluated against a panel of Gram-negative bacterial strains, including both β-lactamase-producing and non-producing organisms.
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| Cell Assay |
In vitro cellular studies are not applicable for temocillin, as it is an antibiotic that targets bacteria, not mammalian cells. Its activity is assessed in bacterial cultures, not in eukaryotic cell lines.
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| Animal Protocol |
In vivo animal experiments are performed in models of bacterial infection. For example, in a murine model of kidney infection, temocillin is administered to infected animals at various doses and frequencies. The primary endpoint is the reduction of bacterial titers in target organs, such as the kidneys.
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| ADME/Pharmacokinetics |
Temocillin disodium salt has a molecular formula of C16H16N2Na2O7S2 and a molecular weight of 458.42 g/mol. It is an organic sodium salt. It is soluble in water and DMSO. For research purposes, it is typically stored as a powder at -20°C. Its purity is generally ≥98%. It appears as an off-white to yellow solid powder.
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| Toxicity/Toxicokinetics |
As a β-lactam antibiotic, temocillin is generally well-tolerated, but can cause hypersensitivity reactions. It is contraindicated in patients with a known allergy to penicillins. Comprehensive toxicological data would be available from its clinical use. For research purposes, standard laboratory safety precautions should be followed.
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| References |
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| Additional Infomation |
Temocillin disodium is an organic sodium salt. It contains Temocillin (2-).
Temocillin disodium salt is also known as Temocillin sodium and has the UNII code 96IIP39ODH. It is a 6-α-methoxy penicillin, which confers resistance to many β-lactamases. It is used as an alternative to carbapenems for treating infections caused by susceptible Gram-negative pathogens. It is supplied as a research-grade compound with high purity. |
| Molecular Formula |
C16H16N2O7S2-2.2[NA+]
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|---|---|
| Molecular Weight |
458.41704
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| Exact Mass |
414.056
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| CAS # |
61545-06-0
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| Related CAS # |
Temocillin;66148-78-5
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| PubChem CID |
11583399
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| Appearance |
Off-white to yellow solid powder
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| Density |
1.6g/cm3
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| Boiling Point |
761.9ºC at 760mmHg
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| Flash Point |
414.6ºC
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| LogP |
1.3
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
29
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| Complexity |
689
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| Defined Atom Stereocenter Count |
3
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| SMILES |
[Na+].[Na+].O=C(C(C(N[C@@]1(C(=O)N2[C@H](C(S[C@H]12)(C)C)C([O-])=O)OC)=O)C1C=CSC=1)[O-]
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| InChi Key |
MRGCZDWBFFUEES-CWBCWDDISA-L
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| InChi Code |
InChI=1S/C16H18N2O7S2.2Na/c1-15(2)9(12(22)23)18-13(24)16(25-3,14(18)27-15)17-10(19)8(11(20)21)7-4-5-26-6-7;;/h4-6,8-9,14H,1-3H3,(H,17,19)(H,20,21)(H,22,23);;/q;2*+1/p-2/t8?,9-,14+,16-;;/m0../s1
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| Chemical Name |
disodium;(2S,5R,6S)-6-[(2-carboxylato-2-thiophen-3-ylacetyl)amino]-6-methoxy-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate
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| Synonyms |
Temocillin sodium; temocillin disodium; 96IIP39ODH; disodium
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~250 mg/mL (~545.35 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (109.07 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1814 mL | 10.9070 mL | 21.8141 mL | |
| 5 mM | 0.4363 mL | 2.1814 mL | 4.3628 mL | |
| 10 mM | 0.2181 mL | 1.0907 mL | 2.1814 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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