| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
TC-O9311 targets GPR139, an orphan G protein-coupled receptor. GPR139 is a relatively poorly characterized receptor that may be involved in metabolic and neurological functions. By activating GPR139, TC-O9311 induces calcium mobilization in receptor-expressing cells. The compound is selective for GPR139 over a panel of 90 diverse targets.
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| ln Vitro |
In vitro, TC-O9311 is a potent GPR139 agonist with an EC50 of 39 nM in CHO-K1 cells expressing human GPR139. It shows no activity against a range of 90 diverse targets. The compound's activity is assessed by measuring calcium mobilization in cells expressing GPR139.
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| ln Vivo |
In vivo, TC-O9311 is used as a research tool to study the function of the orphan receptor GPR139. By activating GPR139, the compound can help elucidate the physiological roles of this receptor in metabolism, neurology, and other systems. Detailed in vivo data are available in research publications.
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| Enzyme Assay |
In vitro receptor binding/functional assays for TC-O9311 measure its activation of GPR139. CHO-K1 cells expressing human GPR139 are treated with serial dilutions of the compound, and calcium mobilization is measured using fluorescent calcium indicators. The EC50 of 39 nM is determined from dose-response curves. Selectivity is confirmed by testing against a panel of 90 diverse targets.
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| Cell Assay |
In vitro cell-based assays for TC-O9311 use CHO-K1 cells expressing human GPR139. Cells are treated with serial dilutions of the compound, and calcium mobilization is measured as a readout of receptor activation. The EC50 for GPR139 activation is calculated from dose-response curves. Cell viability is assessed in parallel.
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| Animal Protocol |
In vivo animal models for TC-O9311 include models where GPR139 function is being studied, such as models of metabolism, neurology, or behavior. The compound is administered via appropriate routes, and its effects on physiological parameters and disease progression are evaluated. Pharmacodynamic studies measure GPR139 activation in target tissues.
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| ADME/Pharmacokinetics |
TC-O9311 has a molecular formula of C20H19N3O4 and a molecular weight of 365.38 g/mol. It is also known as TC-O 9311 and TCO-9311. The compound has an IUPAC name of 2-(3,5-dimethoxybenzoyl)-N-(naphthalen-1-yl)hydrazine-1-carboxamide. It is stored under appropriate conditions for research use and has a purity of ≥98%.
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| Toxicity/Toxicokinetics |
The toxicity profile of TC-O9311 is characterized in preclinical safety studies. As a GPR139 agonist, the compound may affect GPR139-mediated physiological processes. The compound is for research use only and is not approved for clinical use. Appropriate safety precautions should be taken during handling.
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| References | |
| Additional Infomation |
TC-O9311 (TC-O 9311) is a potent agonist of the orphan G protein-coupled receptor GPR139 with an EC50 of 39 nM in CHO-K1 cells expressing human GPR139. It is selective for GPR139 over a panel of 90 diverse targets. TC-O9311 is also known as TCO-9311 and is used as a research tool to study GPR139 function. It is intended for laboratory research use only.
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| Molecular Formula |
C20H19N3O4
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|---|---|
| Molecular Weight |
365.382564783096
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| Exact Mass |
365.138
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| Elemental Analysis |
C, 65.74; H, 5.24; N, 11.50; O, 17.51
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| CAS # |
444932-31-4
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| PubChem CID |
3376937
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| Appearance |
White to off-white solid powder
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| LogP |
4.302
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
27
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| Complexity |
504
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C)C1C=C(C=C(C(NNC(NC2=CC=CC3=CC=CC=C32)=O)=O)C=1)OC
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| InChi Key |
KPTMSQHTGZMEFU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H19N3O4/c1-26-15-10-14(11-16(12-15)27-2)19(24)22-23-20(25)21-18-9-5-7-13-6-3-4-8-17(13)18/h3-12H,1-2H3,(H,22,24)(H2,21,23,25)
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| Chemical Name |
1-[(3,5-dimethoxybenzoyl)amino]-3-naphthalen-1-ylurea
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| Synonyms |
TC-O 9311, TC-O-9311, TC-O9311, TCO 9311, TCO-9311, TCO9311
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~342.11 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.69 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.69 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7369 mL | 13.6844 mL | 27.3688 mL | |
| 5 mM | 0.5474 mL | 2.7369 mL | 5.4738 mL | |
| 10 mM | 0.2737 mL | 1.3684 mL | 2.7369 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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