| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
TBHBA does not have a defined pharmacological target. It is a chemical reagent used in biochemical assays rather than a bioactive molecule. The compound reacts with hydrogen peroxide and 4-aminoantiphenazone in the presence of peroxidase to produce a colored dye. Its primary application is as a detection reagent.
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|---|---|
| ln Vitro |
In vitro, TBHBA is used as a chromogenic reagent in peroxidase-based assays. It reacts with hydrogen peroxide and 4-aminoantiphenazone to produce a quinone-imine dye with high molar absorptivity. The compound is used for the detection and quantification of hydrogen peroxide and peroxidase activity in various biochemical applications.
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| ln Vivo |
In vivo activity of TBHBA has not been characterized, as it is not intended for in vivo use. The compound is a chemical reagent used in laboratory assays. No animal studies have been conducted to evaluate its pharmacological effects or safety.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable to TBHBA, as it is a chromogenic reagent rather than a bioactive molecule. The compound is used in enzymatic assays as a detection reagent for peroxidase activity. The reaction of TBHBA with hydrogen peroxide and 4-aminoantiphenazone in the presence of peroxidase produces a colored product that can be measured spectrophotometrically.
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| Cell Assay |
In vitro cell-based assays are not applicable to TBHBA. The compound is a chemical reagent used in biochemical assays, not for cell-based studies. It is not used in cell culture applications.
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| Animal Protocol |
In vivo animal studies are not applicable to TBHBA. The compound is not intended for administration to animals. It is used exclusively as a laboratory reagent for biochemical assays.
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| ADME/Pharmacokinetics |
Pharmacokinetic (PK) properties of TBHBA have not been characterized, as it is not a therapeutic agent. The compound is a chemical reagent used in laboratory assays. Its PK properties are not relevant to its intended applications.
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| Toxicity/Toxicokinetics |
The toxicity profile of TBHBA has not been characterized. The compound is intended for research use only and is not approved for human therapeutic applications. Standard safety precautions for handling brominated aromatic compounds should be followed.
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| References | |
| Additional Infomation |
TBHBA (2,4,6-Tribromo-3-hydroxybenzoic acid) is a research-grade chromogenic reagent used in peroxidase-based biochemical assays. It is not an approved therapeutic drug. The compound is supplied as a powder with purity ≥98%. Synonyms include 3-Hydroxy-2,4,6-tribromobenzoic acid.
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| Molecular Formula |
C7H3BR3O3
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|---|---|
| Molecular Weight |
374.8089
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| Exact Mass |
371.763
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| CAS # |
14348-40-4
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| PubChem CID |
151915
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| Appearance |
White to off-white solid powder
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| Density |
2.5±0.1 g/cm3
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| Boiling Point |
355.3±42.0 °C at 760 mmHg
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| Melting Point |
145-148 °C(lit.)
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| Flash Point |
168.7±27.9 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.709
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| LogP |
3.5
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
13
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| Complexity |
211
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
YDBHVMTTYXWHLI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C7H3Br3O3/c8-2-1-3(9)6(11)5(10)4(2)7(12)13/h1,11H,(H,12,13)
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| Chemical Name |
2,4,6-tribromo-3-hydroxybenzoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~266.80 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.67 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6680 mL | 13.3401 mL | 26.6802 mL | |
| 5 mM | 0.5336 mL | 2.6680 mL | 5.3360 mL | |
| 10 mM | 0.2668 mL | 1.3340 mL | 2.6680 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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