| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg | |||
| Other Sizes |
| Targets |
The compound is a linker and fluorescent dye, not a drug with a specific biological target. The DBCO group reacts with azide-functionalized molecules via strain-promoted alkyne-azide cycloaddition (SPAAC), a copper-free click chemistry reaction. The TAMRA fluorophore provides a red-fluorescent label for detection and imaging. The hydrophilic PEG4 spacer increases solubility in aqueous media.
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|---|---|
| ln Vitro |
The term "click chemistry" describes a group of reactions that are quick, easy to employ, adaptable, regiospecific, and give large amounts of product. Click chemical reactions are low concentration, low toxicity reactions that work well for cell labeling. DBCO is a great substitute for copper-intolerant applications since it can react with compounds that have been functionalized with azide or biomolecules without the requirement for catalysts to generate stable triazole linkages.
The compound does not possess direct biological activity as it is a linker. Its in vitro utility lies in its ability to form stable conjugates via copper-free click chemistry. The reaction is rapid, selective, and does not require a copper catalyst, making it suitable for use with living systems. It is used for fluorescence labeling, probe development, imaging, and bioconjugation. |
| ln Vivo |
The compound is a chemical reagent and does not have direct in vivo biological activity. Its utility in vivo is as a component of bioconjugates, where it can be used for pretargeted imaging and drug delivery applications. The DBCO group enables bioorthogonal labeling of biomolecules in living systems. The TAMRA fluorophore allows for fluorescent detection in vivo.
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| ADME/Pharmacokinetics |
TAMRA-PEG4-DBCO (CAS 1895849-41-8) has a molecular formula of C54H57N5O10 and a molecular weight of 936.1 g/mol. It is soluble in DMSO, DMF, and DCM. It should be stored at -20°C. The extinction coefficient is 80000. Detailed pharmacokinetic parameters are not applicable as the compound is a linker.
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| Toxicity/Toxicokinetics |
As a chemical linker and fluorescent dye, the compound is not a pharmacologically active compound and does not have direct toxicity. Toxicity would be associated with the final conjugates synthesized using this linker. The PEG component is generally considered biocompatible and non-toxic. No specific toxicity data are provided.
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| References | |
| Additional Infomation |
TAMRA-PEG4-DBCO is a PEG-based fluorescent linker with a DBCO group for copper-free click chemistry. It is used for oligonucleotide labeling and automated DNA sequencing applications. The TAMRA dye is widely used as a dye for oligonucleotide labeling. It is especially useful for probe development, imaging, and bioconjugation.
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| Molecular Formula |
C54H57N5O10
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|---|---|
| Molecular Weight |
936.057894468308
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| Exact Mass |
935.41
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| CAS # |
1895849-41-8
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| PubChem CID |
91757905
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| Appearance |
Light brown to brown solid powder
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| LogP |
4.2
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
21
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| Heavy Atom Count |
69
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| Complexity |
1930
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(C1C=C(C(=O)NCCOCCOCCOCCOCCC(=O)NCCC(N2CC3=CC=CC=C3C#CC3=CC=CC=C23)=O)C=CC=1C1=C2C=CC(N(C)C)=CC2=[O+]C2C=C(N(C)C)C=CC1=2)(=O)[O-]
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| InChi Key |
DTYTXEJLXHBCNC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C54H57N5O10/c1-57(2)41-16-19-44-48(34-41)69-49-35-42(58(3)4)17-20-45(49)52(44)43-18-15-39(33-46(43)54(63)64)53(62)56-24-26-66-28-30-68-32-31-67-29-27-65-25-22-50(60)55-23-21-51(61)59-36-40-11-6-5-9-37(40)13-14-38-10-7-8-12-47(38)59/h5-12,15-20,33-35H,21-32,36H2,1-4H3,(H2-,55,56,60,62,63,64)
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| Chemical Name |
5-[2-[2-[2-[2-[3-[[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]amino]-3-oxopropoxy]ethoxy]ethoxy]ethoxy]ethylcarbamoyl]-2-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~53.42 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (1.34 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0683 mL | 5.3415 mL | 10.6831 mL | |
| 5 mM | 0.2137 mL | 1.0683 mL | 2.1366 mL | |
| 10 mM | 0.1068 mL | 0.5342 mL | 1.0683 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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