| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
T-3364366 targets delta-5 desaturase (D5D / FADS1), a key enzyme in lipid metabolism that catalyzes the conversion of dihomo-γ-linolenic acid (DGLA) to arachidonic acid. It also inhibits D6D with lower potency.
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|---|---|
| ln Vitro |
In vitro, T-3364366 demonstrates potent inhibitory effects on D5D in Sprague-Dawley rat liver microsomes and human HepG2 cells, with IC50 values in the single-digit nanomolar range. It has a D5D Ki < 2.7 nM and a dissociation half-life in excess of 2.0 hours, confirmed in cellular washout assays.
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| ln Vivo |
In vivo, T-3364366 is used to study the role of D5D in lipid metabolism and related diseases. Its long residence time and high potency make it a valuable tool for in vivo pharmacology studies.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for T-3364366 typically involve evaluating its inhibitory activity against purified D5D enzyme using radiometric or chromatographic assays. Ki and IC50 values are determined by measuring the conversion of substrate to product in the presence of varying concentrations of the compound.
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| Cell Assay |
For in vitro cell-based assays, T-3364366 is dissolved in DMSO and applied to cultured hepatocyte cell lines such as HepG2 or RLN-10 at concentrations ranging from 0.1-100 nM. Effects on fatty acid desaturation, lipid composition, and cell viability are assessed after 24-72 hours of treatment.
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| Animal Protocol |
In vivo animal studies commonly use rodent models. T-3364366 is administered orally or intraperitoneally at doses ranging from 0.1-10 mg/kg. Efficacy is evaluated by measuring fatty acid profiles in plasma and tissues, as well as metabolic parameters.
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| ADME/Pharmacokinetics |
T-3364366 has a molecular weight of approximately 400-500 g/mol. It is a reversible, slow-binding inhibitor with long residence time. It is soluble in DMSO.
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| Toxicity/Toxicokinetics |
T-3364366 has a favorable safety profile with no significant toxicity reported at research doses. Its high selectivity for D5D over other desaturases minimizes off-target effects.
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| Additional Infomation |
T-3364366 is used as a tool compound to study the role of D5D in lipid metabolism, inflammation, and metabolic diseases. It has potential therapeutic applications in conditions where modulation of fatty acid desaturation is beneficial, such as cardiovascular disease and metabolic syndrome.
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| Molecular Formula |
C18H16F3N3O3S2
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|---|---|
| Molecular Weight |
443.463151931763
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| Exact Mass |
443.058
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| CAS # |
1356354-09-0
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| PubChem CID |
86685411
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| Appearance |
White to off-white solid powder
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| LogP |
3.5
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
29
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| Complexity |
640
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)NCCSC1=NC2=CSC=C2C(=O)N1C3=CC=C(C=C3)OCC(F)(F)F
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| InChi Key |
BVRXQXNVDLUWMV-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H16F3N3O3S2/c1-11(25)22-6-7-29-17-23-15-9-28-8-14(15)16(26)24(17)12-2-4-13(5-3-12)27-10-18(19,20)21/h2-5,8-9H,6-7,10H2,1H3,(H,22,25)
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| Chemical Name |
N-[2-[4-oxo-3-[4-(2,2,2-trifluoroethoxy)phenyl]thieno[3,4-d]pyrimidin-2-yl]sulfanylethyl]acetamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~281.87 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (4.69 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.69 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2550 mL | 11.2750 mL | 22.5499 mL | |
| 5 mM | 0.4510 mL | 2.2550 mL | 4.5100 mL | |
| 10 mM | 0.2255 mL | 1.1275 mL | 2.2550 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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