| Size | Price | Stock | Qty |
|---|---|---|---|
| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Animal Protocol |
Animals:** Male C57BL/6 mice (8-10 weeks old, 23.5-27.5 g) were housed under controlled temperature and humidity [2]
. - **Surgical Procedure (Aortic Banding):** Mice were anesthetized, and the aortic arch branch was exposed through an incision at the second and third intercostals. The vessel was ligated using a 26G/27G syringe needle placed parallel above the vessel. After needle withdrawal to achieve aortic constriction, the chest was closed. Sham-operated mice underwent the same procedure without vessel ligation. Post-operative pain was managed with 0.1 mL 0.5% bupivacaine injected subcutaneously [2] . - **Drug Administration:** Syringin was dissolved and administered by gavage at doses of 50 mg/kg/day (low-dose) or 100 mg/kg/day (high-dose) for 7 weeks, starting 7 days after surgery. Control groups received equal volumes of normal saline (vehicle) [2] . - **Experimental Groups:** Five groups (n=20 each): sham + vehicle, sham + syringin (100 mg/kg), AB + vehicle, AB + low-dose syringin (50 mg/kg), and AB + high-dose syringin (100 mg/kg) [2] . - **Echocardiography:** At 8 weeks post-surgery, mice were anesthetized with 1.5% isoflurane. Echocardiography was performed using a MyLabTM 30CV cardiovascular ultrasound system with a 10 MHz linear array transducer. LVEDd, IVSD, FS%, and EF% were measured [2] . - **Hemodynamic Measurements:** A microtip catheter transducer was inserted into the right carotid artery and advanced into the left ventricle. dP/dt max and dP/dt min were recorded using a Millar Pressure-Volume System and analyzed by PVAN software [2] . - **Tissue Collection:** Mice were sacrificed by cervical dislocation at 8 weeks post-surgery. Hearts were rapidly excised, weighed, and processed for histological analysis (H&E staining, WGA staining) or frozen for molecular analysis [2] . - **Histological Analysis:** Heart sections were stained with H&E for morphological evaluation. For myocyte cross-sectional area measurement, sections were stained with fluorescein isothiocyanate-conjugated WGA to visualize membranes and DAPI to visualize nuclei. CSA was calculated using Image Pro-Plus 6.0 software [2] . |
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| References |
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| Additional Infomation |
Syringin is a monosaccharide derivative composed of trans-sinopeptidyl alcohol linked to the 1-position of a β-D-glucanose residue via a glycosidic bond. It possesses hepatoprotective, plant metabolic, apoptosis-inducing, autophagy-inducing, anti-inflammatory, neuroprotective, and antidepressant effects. Syringin is a β-D-glucosinolate, a monosaccharide derivative, a primary alcohol, and a dimethoxybenzene. Functionally, it is related to trans-sinopeptidyl alcohol. Syringin has been found in Acanthus ebracteatus, Jasminum mesnyi, and other organisms with relevant data. See also: Codonopsis pilosula root (part).
|
| Molecular Formula |
C17H24O9
|
|---|---|
| Molecular Weight |
372.37
|
| Exact Mass |
372.142
|
| CAS # |
118-34-3
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| PubChem CID |
5316860
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| Appearance |
White to off-white solid powder
|
| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
642.6±55.0 °C at 760 mmHg
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| Melting Point |
192°C
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| Flash Point |
342.4±31.5 °C
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| Vapour Pressure |
0.0±2.0 mmHg at 25°C
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| Index of Refraction |
1.623
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| LogP |
-1.94
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
26
|
| Complexity |
432
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
COC1=CC(=CC(=C1O[C@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CO)O)O)O)OC)/C=C/CO
|
| InChi Key |
QJVXKWHHAMZTBY-GCPOEHJPSA-N
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| InChi Code |
InChI=1S/C17H24O9/c1-23-10-6-9(4-3-5-18)7-11(24-2)16(10)26-17-15(22)14(21)13(20)12(8-19)25-17/h3-4,6-7,12-15,17-22H,5,8H2,1-2H3/b4-3+/t12-,13-,14+,15-,17+/m1/s1
|
| Chemical Name |
(2R,3S,4S,5R,6S)-2-(hydroxymethyl)-6-[4-[(E)-3-hydroxyprop-1-enyl]-2,6-dimethoxyphenoxy]oxane-3,4,5-triol
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| Synonyms |
NSC287441 Eleutheroside B NSC 287441Syringin NSC-287441
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~268.55 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.71 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6855 mL | 13.4275 mL | 26.8550 mL | |
| 5 mM | 0.5371 mL | 2.6855 mL | 5.3710 mL | |
| 10 mM | 0.2686 mL | 1.3428 mL | 2.6855 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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