| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| ln Vitro |
One type of phenol included in certain alcoholic drinks is syringic acid. Moreover, it eliminates (eradicates) the goods that encourage the loss of anthocyanins and other polyphenols [1]. According to research, wine's phenolics may help prevent low-density lipoprotein (LDL) from oxidizing, which is a critical stage in the development of atherosclerotic plaques. High levels of antioxidant activity and the suppression of LDL oxidation have been linked to syringic acid [2].
1. Syringic acid was identified as one of the metabolites generated by the metabolism of Cabernet Sauvignon anthocyanins by human gut microflora in an in vitro incubation system. The system contained gut microflora suspension and anthocyanin extract, and Syringic acid was isolated and confirmed via chromatographic and mass spectrometric methods after incubation [1] 2. As a component in wines, Syringic acid exhibited in vitro antioxidant activity. It could scavenge free radicals (including DPPH and ABTS radicals) and inhibit low-density lipoprotein (LDL) oxidation. Wine samples containing Syringic acid showed free radical scavenging ability and reduced LDL oxidation level (evaluated by oxidized LDL content) [2] |
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| Enzyme Assay |
1. For detecting Syringic acid as a gut microflora metabolite: The in vitro incubation mixture of gut microflora and anthocyanins was pretreated (centrifugation to remove bacterial cells and organic solvent extraction). The pretreated sample was then analyzed using high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS), where characteristic ions of Syringic acid were detected to confirm its existence and content [1]
2. For determining antioxidant activity related to Syringic acid in wines: The DPPH radical scavenging assay involved mixing wine samples (with Syringic acid) with DPPH solution, dark incubation, and absorbance measurement at a specific wavelength to calculate the scavenging rate. The LDL oxidation inhibition assay included incubating LDL with wine samples, adding an oxidant, and monitoring changes in LDL oxidation indices (e.g., conjugated dienes) to assess the inhibition effect [2] |
| References |
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| Additional Infomation |
Syringic acid is a dimethoxybenzene, a 3,5-dimethyl ether derivative of gallic acid. It is a plant metabolite. Syringic acid belongs to the benzoic acid, dimethoxybenzene, and phenolic compounds. Functionally, it is related to gallic acid. It is the conjugate acid of syringate esters. Syringic acid has been reported in peony (Paeonia obovata), rhinacanthus nasutus, and other organisms with relevant data. Syringic acid is a metabolite found or produced in Saccharomyces cerevisiae.
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| Molecular Formula |
C21H38O5SI2
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|---|---|
| Molecular Weight |
426.6944
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| Exact Mass |
198.052
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| CAS # |
530-57-4
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| Related CAS # |
Syringic acid-d6;84759-06-8
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| PubChem CID |
10742
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
379.5±42.0 °C at 760 mmHg
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| Melting Point |
205-209 °C(lit.)
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| Flash Point |
155.0±21.4 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.566
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| LogP |
1.13
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
14
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| Complexity |
191
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JMSVCTWVEWCHDZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H10O5/c1-13-6-3-5(9(11)12)4-7(14-2)8(6)10/h3-4,10H,1-2H3,(H,11,12)
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| Chemical Name |
4-hydroxy-3,5-dimethoxybenzoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~62.5 mg/mL (~315.39 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (10.50 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (10.50 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (10.50 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3436 mL | 11.7181 mL | 23.4362 mL | |
| 5 mM | 0.4687 mL | 2.3436 mL | 4.6872 mL | |
| 10 mM | 0.2344 mL | 1.1718 mL | 2.3436 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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