| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
Sulforhodamine 101 targets astrocytes as a specific marker. The compound is a fluorescent dye that binds to cellular components and allows visualization of astrocytes in tissue sections and cell cultures. Its red fluorescence (excitation max: 586 nm; emission max: 605 nm) enables detection. The compound is used in fluorescence microscopy and cell labeling applications.
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| ln Vitro |
Astrocytes in brainstem regions are more strongly and precisely labeled by Texas Red (Sulforhodamine 101) than are astrocytes in the cortex or hippocampus. Texas Red concentrations should be kept as low as feasible to reduce excitotoxic side effects; alternatively, labeling can be done after the experiment is completed [1].
In vitro, Sulforhodamine 101 is a red fluorescent dye used as a specific marker for astrocytes. It has excitation maximum at 586 nm and emission maximum at 605 nm. The compound is used in fluorescence microscopy for astrocyte labeling and imaging. It is a nonfixable dye. Sulforhodamine 101 is a laser grade dye with purity of 99%. |
| ln Vivo |
Topical treatment of 100 μM or intrahippocampal injection of modest doses of 10 μM Texas Red can produce epileptic activity in vivo [1].
In vivo, Sulforhodamine 101 is used as a fluorescent marker for astrocytes in brain tissue. The compound is used in neuroscience research for astrocyte visualization and study. Its red fluorescence enables detection in tissue sections. Further in vivo studies have evaluated its utility in brain imaging applications. |
| Enzyme Assay |
In vitro fluorescence assays for Sulforhodamine 101 involve measuring its fluorescence properties. Excitation and emission spectra are measured at 586 nm and 605 nm respectively. For astrocyte labeling, cells are incubated with the dye and fluorescence is visualized by microscopy. Dye uptake and retention are assessed. Assays are performed in appropriate buffer systems with positive controls such as known fluorescent dyes.
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| Cell Assay |
In vitro cell-based assays for Sulforhodamine 101 are conducted in astrocyte cultures. Cells are cultured in appropriate media and treated with the dye. Astrocyte labeling efficiency is assessed by fluorescence microscopy. Cell viability is assessed by standard assays. Experiments are performed with appropriate positive and negative controls.
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| Animal Protocol |
Sulforhodamine 101 in vivo studies are conducted in animal models for brain imaging. Animals are injected with Sulforhodamine 101 and brain tissue is examined for astrocyte labeling. Fluorescence is visualized by microscopy. Animals are monitored for clinical signs. Tissues are collected for fluorescence analysis at study endpoints. Studies are conducted in accordance with institutional animal care guidelines.
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| ADME/Pharmacokinetics |
Sulforhodamine 101 (MW 606.71-625.15 g/mol, C31H30N2O7S2) appears as a green to dark green powder to crystal. Its purity is min. 95.0 area% by HPLC. The compound is a red fluorescent dye with excitation at 586 nm and emission at 605 nm. It is a specific marker for astrocytes. Pharmacokinetic properties are not typically characterized as it is a fluorescent dye.
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| Toxicity/Toxicokinetics |
Sulforhodamine 101 is generally considered safe as a fluorescent dye for research use. The compound is widely used in neuroscience research for astrocyte labeling. No significant adverse effects have been reported in the available literature at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
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| References | |
| Additional Infomation |
Sulforhodamine 101 is a red fluorescent dye (excitation max: 586 nm; emission max: 605 nm) used as a specific marker for astrocytes. It is a nonfixable dye used in fluorescence microscopy for astrocyte labeling and imaging. The compound is a laser grade dye with high purity. Its molecular formula is C31H30N2O7S2 with a molecular weight of 606.71-625.15 g/mol. All applications are limited to non-human research use.
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| Molecular Formula |
C31H30N2O7S2
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| Molecular Weight |
606.7091
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| Exact Mass |
606.149
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| CAS # |
60311-02-6
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| PubChem CID |
122180
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| Appearance |
Green to dark green solid powder
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| Density |
1.1982 (rough estimate)
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| Index of Refraction |
1.6900 (estimate)
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| LogP |
4.599
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
42
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| Complexity |
1450
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
COIVODZMVVUETJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C31H30N2O7S2/c34-41(35,36)20-9-10-21(26(17-20)42(37,38)39)27-24-15-18-5-1-11-32-13-3-7-22(28(18)32)30(24)40-31-23-8-4-14-33-12-2-6-19(29(23)33)16-25(27)31/h9-10,15-17H,1-8,11-14H2,(H-,34,35,36,37,38,39)
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| Chemical Name |
2-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)-5-sulfobenzenesulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~82.41 mM)
H2O : ~8.33 mg/mL (~13.73 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (3.43 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (3.43 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6482 mL | 8.2412 mL | 16.4823 mL | |
| 5 mM | 0.3296 mL | 1.6482 mL | 3.2965 mL | |
| 10 mM | 0.1648 mL | 0.8241 mL | 1.6482 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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