| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Sulforaphene targets multiple signaling pathways involved in cancer cell survival and proliferation. It inhibits EGFR (epidermal growth factor receptor), p-ERK1/2 (phosphorylated extracellular signal-regulated kinases 1/2), and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells). By inhibiting these pathways, sulforaphene promotes apoptosis (programmed cell death) and inhibits migration of cancer cells. It may also induce phase II detoxifying enzymes.
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| ln Vitro |
HepG2 and Hep3B cells' vitality can be inhibited and cell fluorescence can be induced by sulforaphane (0-80 μM, 48 hours) [4]. HepG2 and Hep3B cells' radioactive sensitization can be inhibited by sulforaphane (0–40 μM), and SUM159 cell movement and current can be inhibited by sulforaphane (0–10 μM, 24 hours) via blocking Hedgehog signaling [5]. Sulforaphane (5 μM, 24) stimulates radiation-induced cell death and inhibits HCT116 and HT- [4].
Sulforaphene promotes cancer cell apoptosis and inhibits migration via inhibiting EGFR, p-ERK1/2, NF-κB, and other signals. It exhibits an ED50 against velvetleaf seedlings approximately 2 x 10-4 M. It has been shown to decrease the viability of breast cancer cells. Sulforaphene has antioxidant, antiviral, antihypertensive, and antiplatelet aggregation activities. It also exhibits antimicrobial properties. |
| ln Vivo |
In the mouse HCT116 xenograft model, sulforaphane (1 and 5 mg/kg, intraperitoneal injection, daily) suppresses tumor growth [6].
Sulforaphene has been studied in vivo for its role in cancer prevention. It may induce phase II detoxifying enzymes and promote apoptosis in cancer cells. It exhibits antimicrobial properties and may support cardiovascular health. It has been shown to have anti-inflammatory effects. |
| Enzyme Assay |
Sulforaphene is a natural product, and its in vitro activity is assessed in cell-based assays. In a typical assay, cancer cells are treated with varying concentrations of sulforaphene, and the effects on cell viability, apoptosis, and migration are measured. The inhibition of EGFR, p-ERK1/2, and NF-κB signaling is assessed by Western blotting.
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| Cell Assay |
Western Blot analysis [5]
Cell Types: HepG2 and Hep3B cells Tested Concentrations: 0-40 μM Incubation Duration: 48 h Experimental Results: Bax expression increased and Bcl-2 expression diminished. 29 Microtubule polymerization in cells [6]. Inhibits the expression of NF-𝜅B-dependent genes (COX-2, iNos and cyclinD1). Sulforaphene is dissolved in DMSO and applied to cultured cancer cells at concentrations ranging from 1-100 µM. The effect on cell viability is assessed using MTT or similar assays. Apoptosis is measured using flow cytometry or caspase activity assays. Cell migration is assessed using wound healing or transwell assays. The inhibition of signaling pathways is assessed by Western blotting. |
| Animal Protocol |
Animal/Disease Models: Mouse HCT116 xenograft model [6]
Doses: 1 and 5 mg/kg Route of Administration: intraperitoneal (ip) injection, daily Experimental Results: Inhibited tumor growth and increased CDK1, MK2 and p38 phosphorylation. |
| ADME/Pharmacokinetics |
Sulforaphene has a molecular weight of 175.27 g/mol. It is a natural product isolated from radish seeds. The compound is typically stored as a powder at -20°C for long-term stability. Sulforaphene is also known as 莱菔素.
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| Toxicity/Toxicokinetics |
Sulforaphene is considered to have low toxicity. It is a natural product that has been studied for its potential health benefits. No significant toxicity has been reported in preclinical studies.
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| References |
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| Additional Infomation |
According to existing data, sulforaphane has been reported in rhododendron (Thulinella chrysantha), mattiola (Matthiola incana), and radish (Raphanus sativus).
Sulforaphene is a natural product isolated from radish seeds. It promotes cancer cell apoptosis and inhibits migration via inhibiting EGFR, p-ERK1/2, NF-κB, and other signals. Sulforaphene exhibits an ED50 against velvetleaf seedlings approximately 2 x 10-4 M. It has antioxidant, antiviral, antihypertensive, and antiplatelet aggregation activities. Sulforaphene has been studied for its role in cancer prevention. |
| Molecular Formula |
C6H9NOS2
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|---|---|
| Molecular Weight |
175.26
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| Exact Mass |
175.012
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| CAS # |
592-95-0
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| PubChem CID |
6433206
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| Appearance |
Colorless to light yellow liquid
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
362.2±42.0 °C at 760 mmHg
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| Flash Point |
172.8±27.9 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.567
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| LogP |
0.47
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
10
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| Complexity |
182
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(C([H])([H])[H])(/C(/[H])=C(\[H])/C([H])([H])C([H])([H])N=C=S)=O
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| InChi Key |
QKGJFQMGPDVOQE-HWKANZROSA-N
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| InChi Code |
InChI=1S/C6H9NOS2/c1-10(8)5-3-2-4-7-6-9/h3,5H,2,4H2,1H3/b5-3+
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| Chemical Name |
(E)-4-isothiocyanato-1-methylsulfinylbut-1-ene
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| Synonyms |
Sulforaphen; Raphanin; Sulforaphene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~570.55 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.26 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.26 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.26 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.7058 mL | 28.5290 mL | 57.0581 mL | |
| 5 mM | 1.1412 mL | 5.7058 mL | 11.4116 mL | |
| 10 mM | 0.5706 mL | 2.8529 mL | 5.7058 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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