| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg | |||
| 500mg | |||
| Other Sizes |
| Targets |
Sulfanitran targets bacterial dihydropteroate synthase (DHPS). By competitively inhibiting DHPS, it blocks the synthesis of folic acid, which is essential for bacterial growth and replication. This mechanism is the basis for its antibacterial and anticoccidial activity. It also stimulates MRP2 activity.
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|---|---|
| ln Vitro |
In vitro, sulfanitran is an antibacterial and anticoccidial agent. It inhibits bacterial growth by interfering with the synthesis of folic acid. It also stimulates MRP2 activity.
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| ln Vivo |
In vivo, sulfanitran is used as an antibacterial and anticoccidial agent in poultry feeds. It is administered orally in feed. It is effective against coccidiosis and various bacterial infections in poultry.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for sulfanitran typically involve evaluating its inhibitory activity against purified DHPS. The enzyme is incubated with varying concentrations of sulfanitran and its substrate, para-aminobenzoic acid (PABA). The reaction is monitored by measuring the formation of dihydrofolate, and the IC₅₀ is determined.
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| Cell Assay |
For in vitro cell-based assays, sulfanitran is dissolved in DMSO or aqueous buffers and applied to cultured bacterial cells at concentrations ranging from 1-100 µg/mL. The Minimum Inhibitory Concentration (MIC) is determined using standard broth dilution methods. The effects on bacterial growth are assessed.
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| Animal Protocol |
In vivo animal studies for sulfanitran are typically conducted in poultry models of coccidiosis or bacterial infection. Sulfanitran is administered orally in feed at doses ranging from 10-100 mg/kg. Efficacy is evaluated by measuring the reduction in pathogen load, clinical signs, and mortality.
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| ADME/Pharmacokinetics |
Sulfanitran has a molecular formula of C₁₄H₁₃N₃O₅S and a molecular weight of 335.34 g/mol. It is a white to off-white powder. It is soluble in DMSO and is typically stored as a powder at room temperature.
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| Toxicity/Toxicokinetics |
Sulfanitran is considered to have a manageable toxicity profile. As a sulfonamide, it may cause hypersensitivity reactions. Appropriate safety precautions should be followed when handling the compound.
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| Additional Infomation |
N-[4-[(4-nitrophenyl)sulfonamido]phenyl]acetamide is a sulfonamide drug. Sulfamethoxazole is a sulfonamide antibiotic used in poultry farming.
Sulfanitran is a sulfonamide antibacterial and anticoccidial agent used in poultry feeds. It is also known as acetyl sulfanitran. Sulfanitran is approved for veterinary use in some countries. It is supplied by various veterinary and research chemical suppliers for non-human use. |
| Molecular Formula |
C14H13N3O5S
|
|---|---|
| Molecular Weight |
335.33
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| Exact Mass |
335.058
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| CAS # |
122-16-7
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| Related CAS # |
Sulfanitran-13C6;1353867-79-4
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| PubChem CID |
5334
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| Appearance |
White to yellow solid powder
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| Density |
1.5g/cm3
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| Melting Point |
239-240° (Kaufmann); mp 264° (Shepherd)
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| Index of Refraction |
1.664
|
| LogP |
4.104
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
|
| Heavy Atom Count |
23
|
| Complexity |
524
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(C1C([H])=C([H])C(=C([H])C=1[H])N([H])C(C([H])([H])[H])=O)(N([H])C1C([H])=C([H])C(=C([H])C=1[H])[N+](=O)[O-])(=O)=O
|
| InChi Key |
GWBPFRGXNGPPMF-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C14H13N3O5S/c1-10(18)15-11-4-8-14(9-5-11)23(21,22)16-12-2-6-13(7-3-12)17(19)20/h2-9,16H,1H3,(H,15,18)
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| Chemical Name |
N-[4-[(4-nitrophenyl)sulfamoyl]phenyl]acetamide
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| Synonyms |
NSC-217299; NSC 217299; Sulfanitran
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 22 mg/mL (~65.61 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9821 mL | 14.9107 mL | 29.8214 mL | |
| 5 mM | 0.5964 mL | 2.9821 mL | 5.9643 mL | |
| 10 mM | 0.2982 mL | 1.4911 mL | 2.9821 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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