| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Sulfabromomethazine targets bacterial dihydropteroate synthase (DHPS), a key enzyme in the folic acid synthesis pathway. By competitively inhibiting DHPS, it blocks the synthesis of folic acid, which is essential for bacterial growth and replication. This mechanism is the basis for its antibacterial activity.
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| ln Vitro |
In vitro, sulfabromomethazine is a broad-spectrum antibiotic that is effective against a wide range of bacterial infections. It is a long-acting sulfonamide. Its antibacterial activity is well-characterized.
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| ln Vivo |
In vivo, sulfabromomethazine is used in veterinary medicine for the treatment of coccidiosis and various bacterial infections in poultry, swine, and cattle. It is administered orally or in feed. It is effective against a variety of pathogens.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for sulfabromomethazine typically involve evaluating its inhibitory activity against purified DHPS. The enzyme is incubated with varying concentrations of sulfabromomethazine and its substrate, para-aminobenzoic acid (PABA). The reaction is monitored by measuring the formation of dihydrofolate, and the IC₅₀ is determined.
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| Cell Assay |
For in vitro cell-based assays, sulfabromomethazine is dissolved in DMSO or aqueous buffers and applied to cultured bacterial cells at concentrations ranging from 1-100 µg/mL. The Minimum Inhibitory Concentration (MIC) is determined using standard broth dilution methods. The effects on bacterial growth are assessed.
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| Animal Protocol |
In vivo animal studies for sulfabromomethazine are typically conducted in poultry, swine, or cattle models of coccidiosis or bacterial infection. Sulfabromomethazine is administered orally or in feed at doses ranging from 10-100 mg/kg. Efficacy is evaluated by measuring the reduction in pathogen load, clinical signs, and mortality.
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| ADME/Pharmacokinetics |
Sulfabromomethazine has a molecular formula of C₁₂H₁₃BrN₄O₂S and a molecular weight of 357.23 g/mol. It is a white to off-white powder. It is soluble in DMSO and is typically stored as a powder at -20°C.
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| Toxicity/Toxicokinetics |
Sulfabromomethazine is considered to have a manageable toxicity profile. As a sulfonamide antibiotic, it may cause hypersensitivity reactions, hematological toxicities, and renal toxicity. Appropriate safety precautions should be followed when handling the compound.
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| Additional Infomation |
Sulfobromodimethylamine is a sulfonamide drug composed of 5-bromo-4,6-dimethylpyrimidine and a 4-aminobenzenesulfonamide group at the 2-position. It is a long-acting derivative of sulfadiazine and is used in poultry, pig, and cattle farming to treat coccidiosis and various bacterial infections. It is an antibacterial agent with similar functions to sulfadiazine. Sulfobromodimethylamine is a long-acting derivative of sulfadiazine and is used in poultry, pig, and cattle farming to treat coccidiosis and various bacterial infections. See also: Sodium sulfadiazine (note moved to).
Sulfabromomethazine is a long-acting sulfonamide antibiotic used in veterinary medicine for the treatment of coccidiosis and bacterial infections. It is also known as Sulfabrom. Sulfabromomethazine is approved for veterinary use in some countries. It is supplied by various veterinary and research chemical suppliers for non-human use. |
| Molecular Formula |
C12H13BRN4O2S
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|---|---|
| Molecular Weight |
357.22
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| Exact Mass |
355.994
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| Elemental Analysis |
C, 40.35; H, 3.67; Br, 22.37; N, 15.68; O, 8.96; S, 8.97
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| CAS # |
116-45-0
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| Related CAS # |
Sulfabrom-d4;2374858-36-1
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| PubChem CID |
8310
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| Appearance |
Orange to light red solid powder
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| Density |
1.653g/cm3
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| Boiling Point |
568.3ºC at 760 mmHg
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| Flash Point |
297.5ºC
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| Vapour Pressure |
6.23E-13mmHg at 25°C
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| Index of Refraction |
1.663
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| LogP |
3.973
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
20
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| Complexity |
408
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=S(C1=CC=C(N)C=C1)(NC2=NC(C)=C(Br)C(C)=N2)=O
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| InChi Key |
KWXCNODTHBHSIQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H13BrN4O2S/c1-7-11(13)8(2)16-12(15-7)17-20(18,19)10-5-3-9(14)4-6-10/h3-6H,14H2,1-2H3,(H,15,16,17)
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| Chemical Name |
4-amino-N-(5-bromo-4,6-dimethylpyrimidin-2-yl)benzenesulfonamide
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| Synonyms |
NSC 5874; Bromosulfamethazine; 5-Bromosulfamethazine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~279.93 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (4.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.67 mg/mL (4.67 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.67 mg/mL (4.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7994 mL | 13.9970 mL | 27.9940 mL | |
| 5 mM | 0.5599 mL | 2.7994 mL | 5.5988 mL | |
| 10 mM | 0.2799 mL | 1.3997 mL | 2.7994 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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