| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
The primary biological target is Mycobacterium tuberculosis; the specific molecular target has not been fully defined. Stigmasta-4,22-dien-3-one exhibits antitubercular activity and cytotoxicity against HT1080 tumor cells, possibly through membrane disruption or steroid-mediated signaling.
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|---|---|
| ln Vitro |
In vitro, Stigmasta-4,22-dien-3-one shows antitubercular activity and cytotoxicity against the human HT1080 tumor cell line with an IC50 of 0.3 mM.
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| ln Vivo |
No dedicated in vivo pharmacokinetic or efficacy datasets are reported in the available public records for Stigmasta-4,22-dien-3-one. It is a steroid with in vitro antitubercular and cytotoxic activities.
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| Enzyme Assay |
Antitubercular susceptibility assay: culture Mycobacterium tuberculosis in the presence of serially diluted Stigmasta-4,22-dien-3-one, and determine MIC values by broth microdilution or agar proportion methods.
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| Cell Assay |
Cell-based cytotoxicity assay: treat HT1080 or other cancer cell lines with serially diluted Stigmasta-4,22-dien-3-one for 48–72 h, measure cell viability by MTT, and calculate IC50.
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| Animal Protocol |
In vivo study design (as applicable for an antitubercular agent): administer Stigmasta-4,22-dien-3-one to mice infected with M. tuberculosis, measure bacterial burden in lungs and spleen, and assess survival and histopathology.
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| References | |
| Additional Infomation |
Stigmaster-4,22-dien-3-one is a steroidal compound derived from the hydrogenation of stigmasterane. It has been reported to exist in Rhinacanthus nasutus, Pellia epiphylla, and other organisms with relevant data.
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| Molecular Formula |
C29H46O
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|---|---|
| Molecular Weight |
410.67494
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| Exact Mass |
410.355
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| CAS # |
20817-72-5
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| Related CAS # |
(22E,24R)-Stigmasta-4,22-dien-3-one;55722-32-2
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| PubChem CID |
6442194
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| Appearance |
White to off-white solid
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| Source |
Originated from plants: Leguminosae Erythrina variegata Linn.
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| LogP |
8.009
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| tPSA |
17.07
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
30
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| Complexity |
714
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| Defined Atom Stereocenter Count |
8
|
| SMILES |
CCC(C(C)C)/C=C/C(C1C2(C)C(C3C(CC2)C2(C)C(=CC(=O)CC2)CC3)CC1)C
|
| InChi Key |
MKGZDUKUQPPHFM-LPJPOILFSA-N
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| InChi Code |
InChI=1S/C29H46O/c1-7-21(19(2)3)9-8-20(4)25-12-13-26-24-11-10-22-18-23(30)14-16-28(22,5)27(24)15-17-29(25,26)6/h8-9,18-21,24-27H,7,10-17H2,1-6H3/b9-8+/t20-,21-,24+,25-,26+,27+,28+,29-/m1/s1
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| Chemical Name |
(8S,9S,10R,13R,14S,17R)-17-[(E,2R,5S)-5-ethyl-6-methylhept-3-en-2-yl]-10,13-dimethyl-1,2,6,7,8,9,11,12,14,15,16,17-dodecahydrocyclopenta[a]phenanthren-3-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Ethanol: ~25 mg/mL (~60.9 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.09 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of EtOH stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4350 mL | 12.1752 mL | 24.3505 mL | |
| 5 mM | 0.4870 mL | 2.4350 mL | 4.8701 mL | |
| 10 mM | 0.2435 mL | 1.2175 mL | 2.4350 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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