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| ln Vitro |
On MDA-MB-231 cells, STAT3-IN-11 (20 μM, 48 h) had a 97.86% inhibitory impact [1]. At IC50 values of 6.01 μM (MDA-MB-231) and 7.02 μM (HepG2, A549), STAT3-IN-11 (0-30 μM, 48 h) inhibits a range of cancer cells, whereas at IC50 values of 26.54 μM (MDA-MB-10A), 26.69 μM (PBMC), and 12.52 μM (HFL-1) it inhibits normal human cells [1]. The phosphorylation of STAT3 (induced by IL-6 in MDA-MB-231) at pTyr705 is dose-dependently inhibited by STAT3-IN-11 (2.5-10 μM, 6 hours) [1]. In a concentration-dependent manner, STAT3-IN-11 (2.5-10 μM, 6 h) suppresses the expression of STAT3 downstream genes, namely Mcl-1 and Survivin [1]. The phosphorylation of canonical upstream kinases of STAT3 (p-JAK2 and p-Src) and STAT1 (a STAT isoform) is unaffected by STAT3-IN-11 (2.5–10 μM, 6 hours) [1]. Apoptosis can be induced dose-wise by STAT3-IN-11 (2.5-10 μM, 48 h) [1].
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| Cell Assay |
Cytotoxicity assay [1]
Cell Types: MDA-MB-231 Cell Tested Concentrations: 20 μM Incubation Duration: 48 hrs (hours) Experimental Results: Anti-proliferative activity reached 97.86%. Cytotoxicity assay[1] Cell Types: MDA-MB231, A549, MDA-MB-10A, PBMC and HFL-1 Cell Tested Concentrations: 20 μM Incubation Duration: 48 hrs (hours) Experimental Results: Inhibition with IC50 values of 6.01, 7.20, 7.02, 26.54 25.69 and 12.52 μM for MDA-MB231, A549, MDA-MB-10A, PBMC and HFL-1 cells, respectively. Western Blot Analysis[1] Cell Types: MDA-MB-231 Cell Tested Concentrations: 2.5, 5 and 10 μM Incubation Duration: 6 hrs (hours) Experimental Results: diminished the level of p-STAT3 at pTyr705 site in a concentration-dependent manner. Western Blot Analysis [1] Cell Types: MDA-MB-231 Cell Tested Concentrations: 2.5, 5 and 10 μM Incubation Duration: 6 hrs (hours) Experimental Results: STAT3 downstream gene levels were down-regulated. Apoptosis analysis [1] Cell Types: MDA-MB-231 Cell Tested Concentrations: 5, 10 and 15 μM Incubation Duration: 48 hrs (hours) Experimental Results: At a concentration of 15 μM, the percentage of apoptotic cells was 24.4%. |
| References |
| Molecular Formula |
C20H17NO4
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|---|---|
| Molecular Weight |
335.353285551071
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| Exact Mass |
335.115
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| CAS # |
2503096-50-0
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| PubChem CID |
164517204
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| Appearance |
Yellow to orange solid powder
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| LogP |
3.3
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
25
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| Complexity |
589
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(NCCC1=C(C)C(=O)C2=C(C1=O)C(O)=CC=C2)(=O)C1=CC=CC=C1
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| InChi Key |
XLSYRTJOLUDGSR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H17NO4/c1-12-14(10-11-21-20(25)13-6-3-2-4-7-13)19(24)17-15(18(12)23)8-5-9-16(17)22/h2-9,22H,10-11H2,1H3,(H,21,25)
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| Chemical Name |
N-[2-(8-hydroxy-3-methyl-1,4-dioxonaphthalen-2-yl)ethyl]benzamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~298.20 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (7.45 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9820 mL | 14.9098 mL | 29.8196 mL | |
| 5 mM | 0.5964 mL | 2.9820 mL | 5.9639 mL | |
| 10 mM | 0.2982 mL | 1.4910 mL | 2.9820 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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