| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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| ln Vitro |
Stachyine's anti-hypertrophic action may be associated with its ability to suppress the NF-κB signaling pathway. The levels of NF-κB protein in the nucleus and p-IκB protein in the cytoplasm are both markedly inhibited by strepthydrine intervention [1]. After stachydrine treatment, human umbilical vein endothelial cells exhibit a decrease in tissue factor mRNA. Stachyrine has the ability to mitigate the effects of hypoxia and reoxygenation-induced increases in LDH activity and decreases in human umbilical vein endothelial cell viability [2]. Stachydrine-treated human prostate cancer cells (PC-3 and LNcaP) showed a dose-dependent reduction in both protein levels and mRNA expression [3].
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|---|---|
| ln Vivo |
Stachyhydrine treatment can decrease the expression of PERK, CHOP, and caspase-3 in the endoplasmic reticulum stress-related apoptotic pathway [5]. Stachyhydrine can also potentially protect against β-adrenergic receptor-induced Ca2+ mishandling [4].
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| References |
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| Additional Infomation |
L-proline betaine is an amino acid betaine that is L-proline zwitterion in which both of the hydrogens attached to the nitrogen are replaced by methyl groups. It has a role as a food component, a plant metabolite and a human blood serum metabolite. It is a N-methyl-L-alpha-amino acid, an alkaloid and an amino-acid betaine. It is functionally related to a L-prolinium. It is a conjugate base of a N,N-dimethyl-L-prolinium. It is an enantiomer of a D-proline betaine.
Stachydrine is a metabolite found in or produced by Escherichia coli (strain K12, MG1655). Stachydrine has been reported in Leonurus japonicus, Achillea setacea, and other organisms with data available. |
| Molecular Formula |
C7H14NO2
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|---|---|
| Molecular Weight |
144.1916
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| Exact Mass |
143.094
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| CAS # |
471-87-4
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| Related CAS # |
Stachydrine hydrochloride;4136-37-2
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| PubChem CID |
115244
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| Appearance |
White to off-white solid powder
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| Density |
1.1095 (rough estimate)
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| Boiling Point |
261.28°C (rough estimate)
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| Melting Point |
235°C
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| Index of Refraction |
1.4150 (estimate)
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| LogP |
-2.97
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
10
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| Complexity |
148
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C[N+]1(CCC[C@H]1C(=O)[O-])C
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| InChi Key |
CMUNUTVVOOHQPW-LURJTMIESA-N
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| InChi Code |
InChI=1S/C7H13NO2/c1-8(2)5-3-4-6(8)7(9)10/h6H,3-5H2,1-2H3/t6-/m0/s1
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| Chemical Name |
(2S)-1,1-dimethylpyrrolidin-1-ium-2-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~698.42 mM)
DMSO : ~100 mg/mL (~698.42 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (17.46 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (17.46 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (17.46 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 110 mg/mL (768.26 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.9353 mL | 34.6765 mL | 69.3529 mL | |
| 5 mM | 1.3871 mL | 6.9353 mL | 13.8706 mL | |
| 10 mM | 0.6935 mL | 3.4676 mL | 6.9353 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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