| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 1g |
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| 5g |
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| 25g |
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| Other Sizes |
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| ln Vitro |
Protein synthesis in cells and E is specifically inhibited by spectinomycin dihydrochloride. Coli extracts: 50 μg/mL of streptomycin dihydrochloride quickly and reversibly inhibits E. coli development and stops the incorporation of amino acids right away [1]. With a maximal inhibition rate of 70-80% in E, spectinomycin diHClide (1 μg/mL or 3 μM) inhibits peptide synthesis directed by endogenous messenger RNA or MS-2 phage RNA. extracted from Coli[1][1]. Spectinomycin dihydrochloride prevents elongation factor G from attaching to the ribosome, hence preventing peptidyl tRNA from translocating from the A site to the P site [2]. Reagents G1064 and 01192 in 16S rRNA are particularly interacting with and may be rendered inactive by streptinomycin diHClide [2]. Due to its dependence on pH variations and Mg2+ concentration, spectinomycin diHClide shows inhibition of splicing, which may indicate electrostatic interactions with intronic RNA [3].
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|---|---|
| ln Vivo |
It has been demonstrated that in healthy chicks, spectinomycin dihydrochloride (20 mg/kg; IM; 20-100 mg/kg; 9 days) is safe [4]. The majority of the dose of specinomycin dihydrochloride (10 mg/kg; intravenous injection; single dose) is excreted unchanged into the urine by the kidneys [5]. Pharmacokinetics of spectinomycin hydrochloride in rats [5] Parameter C0 (μg/mL) AUC0-∞ (μg·h/mL) Vd (L/kg) CL (L/h/kg) MRT (h) T1/2α (h) T1/2β (h) fe CLrenal (L/h/kg) Eratio non-compartment analysis 44.3 16.8 0.756 0.602 0.757 / / 0.553 0.359 1.00 Three-compartment model 37.8 15.7 0.747 0.649 1.11 / 0.237 0.754 19.5 / /
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| Animal Protocol |
Animal/Disease Models: Arbor Acres plus broiler chicken (15 days old) [4]
Doses: 20 mg/kg, 60 mg/kg, 100 mg/kg Route of Administration: intramuscularinjection (chest muscle); 9 Day Experimental Results: Complete blood count, biochemical parameters, histopathology, clinical signs, weight gain and feed conversion ratio (FCR) demonstrated biosafety of 20 mg/kg. Produces mild toxicity at 60 mg/kg. |
| References | |
| Additional Infomation |
Spectinomycin dihydrochloride is a hydrochloride obtained by combining spectinomycin with two molar equivalents of hydrochloric acid. An antibiotic that is active against gram-negative bacteria and used (as its pentahydrate) to treat gonorrhea. It has a role as an antibacterial drug and an antimicrobial agent. It contains a spectinomycin(2+).
Spectinomycin Hydrochloride is the hydrochloride salt form of spectinomycin, an aminocyclitol antibiotic derived from Streptomyces spectabilis with antibacterial property. Spectinomycin hydrochloride binds to the bacterial 30S ribosomal subunit, thereby inhibiting peptide elongation and protein synthesis, consequently leading to bacterial cell death. An antibiotic produced by Streptomyces spectabilis. It is active against gram-negative bacteria and used for the treatment of gonorrhea. See also: Spectinomycin Hydrochloride (annotation moved to). |
| Molecular Formula |
C14H26CL2N2O7
|
|---|---|
| Molecular Weight |
405.2714
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| Exact Mass |
404.111
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| CAS # |
21736-83-4
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| Related CAS # |
Spectinomycin dihydrochloride pentahydrate;22189-32-8;Spectinomycin;1695-77-8
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| PubChem CID |
64740
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| Appearance |
White to off-white solid powder
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| Density |
1.43g/cm3
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| Boiling Point |
583.1ºC at 760 mmHg
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| Melting Point |
194ºC
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| Flash Point |
306.4ºC
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| Vapour Pressure |
5.05E-16mmHg at 25°C
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| Index of Refraction |
1.586
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
25
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| Complexity |
478
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| Defined Atom Stereocenter Count |
9
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| SMILES |
C[C@@H]1CC(=O)[C@]2([C@@H](O1)O[C@@H]3[C@H]([C@@H]([C@@H]([C@@H]([C@H]3O2)NC)O)NC)O)O.Cl.Cl
|
| InChi Key |
FOGDPGQSHLLYIK-XYQGXRRISA-N
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| InChi Code |
InChI=1S/C14H24N2O7.2ClH/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;;/h5,7-13,15-16,18-20H,4H2,1-3H3;2*1H/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;/m1../s1
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| Chemical Name |
(1R,3S,5R,8R,10R,11S,12S,13R,14S)-8,12,14-trihydroxy-5-methyl-11,13-bis(methylamino)-2,4,9-trioxatricyclo[8.4.0.03,8]tetradecan-7-one;dihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 100 mg/mL (~246.75 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (123.37 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4675 mL | 12.3375 mL | 24.6749 mL | |
| 5 mM | 0.4935 mL | 2.4675 mL | 4.9350 mL | |
| 10 mM | 0.2467 mL | 1.2337 mL | 2.4675 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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