| Size | Price | |
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| Other Sizes |
| Targets |
SPDP Crosslinker functions as a chemical crosslinking reagent, not a drug targeting a biological receptor. It contains an NHS ester that reacts with primary amines (e.g., on lysine residues of proteins) and a pyridyldithiol group that reacts with sulfhydryl groups (e.g., on cysteine residues). This allows for the formation of stable, cleavable linkages between two molecules, enabling reversible conjugation.
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| ln Vitro |
SPDP was used to activate equal volumes of anti-CD11c and anti-CTLA-4 Ab (in borate-buffered saline; pH 8.5).
In vitro, SPDP Crosslinker is used to conjugate proteins or other molecules containing amines and sulfhydryls. It is a heterobifunctional crosslinker that can be used to prepare antibody-drug conjugates (ADCs) by linking antibodies to cytotoxic drugs via a cleavable disulfide bond. Its reactivity with amines and thiols makes it a versatile tool for bioconjugation. |
| ln Vivo |
In vivo, SPDP Crosslinker is not used as a therapeutic agent. It is a chemical reagent used in the preparation of bioconjugates, such as ADCs, which are then used therapeutically. The crosslinker itself is not administered to living organisms; only the final conjugated product is administered.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable for SPDP Crosslinker, as it is a chemical crosslinking reagent, not a compound that targets specific enzymes or receptors. Its utility is based on its chemical reactivity with amines and thiols, which can be assessed by simple chemical assays (e.g., Ellman's test for thiol modification).
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| Cell Assay |
In vitro cellular assays are not typically performed for SPDP Crosslinker, as it is a chemical reagent used for bioconjugation. Its effects are studied in the context of protein conjugation and the preparation of bioconjugates, rather than in cell-based pharmacological models. However, cell-based assays may be used to test the activity of the final conjugate.
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| Animal Protocol |
In vivo animal models are not applicable for SPDP Crosslinker itself, as it is not a therapeutic agent. However, bioconjugates prepared using SPDP Crosslinker (e.g., ADCs) may be evaluated in animal models for their therapeutic efficacy, pharmacokinetics, and toxicity. The crosslinker's properties influence the stability and release of the drug.
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| ADME/Pharmacokinetics |
SPDP Crosslinker has a molecular formula of C12H12N2O4S2 and a molecular weight of 312.37 g/mol. Its chemical name is N-succinimidyl 3-(2-pyridyldithio)propionate. It is a heterobifunctional crosslinker and is typically stored as a powder at -20°C. It is soluble in DMSO and other organic solvents, and should be handled under anhydrous conditions.
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| Toxicity/Toxicokinetics |
SPDP Crosslinker is a chemical reagent and is considered hazardous. It can cause skin and eye irritation and is harmful if swallowed. Appropriate safety precautions, including the use of personal protective equipment (gloves, goggles), should be followed when handling this compound. It is not intended for human therapeutic use.
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| References |
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| Additional Infomation |
SPDP Crosslinker is a heterobifunctional crosslinker featuring an NHS ester and a cleavable disulfide bond. It is used for amine-to-sulfhydryl conjugation in the preparation of antibody-drug conjugates (ADCs) and other bioconjugates. The compound is for research use only and is not approved for human therapeutic use.
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| Molecular Formula |
C12H12N2O4S2
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|---|---|
| Molecular Weight |
312.3647
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| Exact Mass |
312.023
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| CAS # |
68181-17-9
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| PubChem CID |
100682
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
465.9±55.0 °C at 760 mmHg
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| Melting Point |
84-86 °C(lit.)
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| Flash Point |
235.6±31.5 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.650
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| LogP |
0.99
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
20
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| Complexity |
376
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JWDFQMWEFLOOED-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H12N2O4S2/c15-10-4-5-11(16)14(10)18-12(17)6-8-19-20-9-3-1-2-7-13-9/h1-3,7H,4-6,8H2
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 3-(pyridin-2-yldisulfanyl)propanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. (3). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~155 mg/mL (~496.22 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.66 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.66 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2014 mL | 16.0072 mL | 32.0143 mL | |
| 5 mM | 0.6403 mL | 3.2014 mL | 6.4029 mL | |
| 10 mM | 0.3201 mL | 1.6007 mL | 3.2014 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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