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| Other Sizes |
| Targets |
The molecular targets of sodium copper chlorophyllin B are not specific protein targets but rather its antioxidant and photoprotective properties. The compound acts as an antioxidant by scavenging free radicals and reducing oxidative stress. It may also modulate inflammatory pathways by reducing the production of pro-inflammatory mediators. Its photoprotective effects are attributed to its ability to absorb light and protect cells from UV-induced damage. The compound's copper content may also contribute to its biological activities through the modulation of copper-dependent enzymes and signaling pathways.
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| ln Vitro |
Chlorophyll B sodium copper demonstrated influenza virus adsorption at 200 µM (P<0.05). The IC50 and 50% cytotoxic concentration (CC50) of sodium copper chlorophyllin are 50 to 100 µM and 200 to 400 µM, respectively. Sodium chlorophyll B copper also decreased HIV adsorption at 2.5 mM (P<0.05). The IC50 and CC50 of sodium copper chlorophyllin are 50 to 100 µM and 200 to 400 µM respectively[1].
In vitro, sodium copper chlorophyllin B exhibits potent antioxidant activity by scavenging free radicals and reducing oxidative stress. It has been shown to have anti-inflammatory effects by reducing the production of pro-inflammatory mediators in cell-based assays. The compound also demonstrates photoprotective activity by protecting cells from UV-induced damage. Its wound healing properties have been observed in cell-based assays, where it promotes cell proliferation and migration. Sodium copper chlorophyllin B has also been studied for its anticancer potential, with evidence suggesting that it may inhibit cell proliferation and induce apoptosis in cancer cells. |
| ln Vivo |
In vivo, sodium copper chlorophyllin B has been studied for its wound healing, anti-inflammatory, and photoprotective properties. It has been shown to promote wound healing in animal models by reducing inflammation and promoting tissue regeneration. The compound's anti-inflammatory effects have been observed in various models of inflammation, where it reduces the production of pro-inflammatory mediators. Its photoprotective effects have been demonstrated in animal models of UV-induced skin damage, where it reduces erythema and oxidative stress. Sodium copper chlorophyllin B has also been studied for its potential anticancer activity in animal models.
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| Enzyme Assay |
Cell-free assays for sodium copper chlorophyllin B typically involve assessing its antioxidant activity. A common protocol for DPPH radical scavenging assay involves preparing a DPPH solution in methanol and incubating it with various concentrations of the compound at room temperature for 30 minutes. The absorbance is measured at 517 nm, and the percentage of radical scavenging activity is calculated. For ABTS radical cation decolorization assay, ABTS is oxidized with potassium persulfate to generate the ABTS radical cation, which is then incubated with the compound. The decrease in absorbance at 734 nm is measured. The IC50 value is determined by plotting the percentage of scavenging against the compound concentration.
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| Cell Assay |
For in vitro cellular experiments, cells (e.g., fibroblasts, keratinocytes, cancer cell lines) are cultured in appropriate media and treated with sodium copper chlorophyllin B at various concentrations (typically 1-100 uM). For antioxidant studies, cells are exposed to oxidative stress (e.g., H2O2, UV irradiation) in the presence or absence of the compound, and oxidative stress markers (e.g., ROS, MDA) are measured. For wound healing studies, cell proliferation and migration are assessed using scratch or Transwell assays. For anti-inflammatory studies, cells are stimulated with LPS, and cytokine production is measured by ELISA. Cell viability is assessed using MTT or CCK-8 assays.
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| Animal Protocol |
In vivo animal experiments with sodium copper chlorophyllin B typically involve topical or oral administration in rodent models. For wound healing studies, full-thickness skin wounds are created on the backs of animals, and the compound is applied topically. Wound closure is monitored over time, and tissue samples are collected for histopathological examination. For photoprotection studies, animals are exposed to UV irradiation after topical application of the compound, and skin damage is assessed by measuring erythema, edema, and oxidative stress markers. For anti-inflammatory studies, animals are treated with the compound before or after induction of inflammation, and inflammatory markers are measured.
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| ADME/Pharmacokinetics |
Sodium copper chlorophyllin B is a water-soluble compound with a molecular weight of approximately 724 g/mol. As a chlorophyll derivative, it is expected to have limited oral bioavailability due to its hydrophilic nature and large molecular size. The compound is typically administered topically or by injection in preclinical studies. Its pharmacokinetic properties, including absorption, distribution, metabolism, and excretion, would need to be characterized for systemic administration. The compound is stable as a powder and should be stored at room temperature in a dry, dark place.
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| Toxicity/Toxicokinetics |
The toxicity profile of sodium copper chlorophyllin B has been well characterized due to its widespread use as a food additive and in cosmetic products. It is generally recognized as safe (GRAS) by regulatory authorities at the levels used in food and cosmetics. However, high doses may cause gastrointestinal discomfort or other mild adverse effects. The compound has low acute toxicity and is not considered genotoxic or carcinogenic. It should be handled with standard laboratory precautions and is intended for research use.
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| References | |
| Additional Infomation |
Sodium copper chlorophyllin B (CAS 28302-36-5) is a water-soluble, semi-synthetic derivative of chlorophyll. It is used as a food coloring agent (E141) and in various health and cosmetic products due to its antioxidant and photoprotective properties. The compound exhibits antioxidant, anti-inflammatory, photoprotective, and wound healing activities. It is also studied for its potential anticancer activity. Sodium copper chlorophyllin B is generally recognized as safe and is available as a research compound for studying its biological activities and potential therapeutic applications. It is not approved for therapeutic use.
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| Molecular Formula |
C34H29CUN4NA3O7
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|---|---|
| Molecular Weight |
738.14
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| CAS # |
28302-36-5
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| Appearance |
Black green to black solid powder
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| Boiling Point |
790ºC at 760mmHg
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| Flash Point |
431.6ºC
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~10 mg/mL (~13.55 mM)
DMSO : ~6.67 mg/mL (~9.04 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.67 mg/mL (0.91 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 6.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.67 mg/mL (0.91 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 6.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 0.67 mg/mL (0.91 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 20 mg/mL (27.10 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3548 mL | 6.7738 mL | 13.5476 mL | |
| 5 mM | 0.2710 mL | 1.3548 mL | 2.7095 mL | |
| 10 mM | 0.1355 mL | 0.6774 mL | 1.3548 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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