| Size | Price | Stock | Qty |
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| 10g |
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| 25g |
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| Other Sizes |
| Targets |
Sodium catechol sulfate (Tiron) is a potent antioxidant that acts as a scavenger of reactive oxygen species (ROS), specifically hydroxyl radicals and superoxide anions. It functions as a metal chelator, binding to various metal ions, which prevents them from catalyzing the formation of free radicals. By chelating metals and scavenging ROS, it protects cells from oxidative damage.
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| ln Vitro |
Chinese rat hamster V79 cell scaffolds are shielded from H2O2-induced cytotoxicity by titan (10 mM) [1]. In superoxygen scaffolds, tiron (0–20 mM) guards against metal-mediated strand breaking of supercoiled DNA scaffolds [1]. In freshly transplanted cells, titan (50 nM-200 nM, 48 h) lowers osteopontin [3]. In freshly transplanted cells, titan (50 nM-200 nM, 48 h) lowers osteopontin [3]. In HDF, tiron (0.2 mM, 2 hours) suppresses the overexpression of MMP-1 and MMP-3 caused by UVB [4]. The percentage of PT4 cells in S phase and G2/M phase is increased by tiron (0.7 mM, 48 hours) [5].
In vitro, Sodium catechol sulfate exhibits strong antioxidant activity by scavenging free radicals and chelating metal ions. It is used as a tool to study oxidative stress and the effects of ROS in various cell culture models. The compound's ability to penetrate cells makes it suitable for studying intracellular oxidative damage. |
| ln Vivo |
In a mouse airway model, tiron (200 mg/kg, gavage) enhances induction and oxidation [2]. Tiron attenuates STZ induction (300 mg/kg, ip, once daily for one week).
In vivo, Sodium catechol sulfate is an orally bioactive antioxidant that has been shown to protect against oxidative stress in animal models. It is used in research to study the role of oxidative stress in various disease conditions. Its metal-chelating properties also make it useful for studying metal toxicity. |
| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable for Sodium catechol sulfate, as its primary mechanism of action is as an antioxidant and metal chelator rather than a specific enzyme or receptor inhibitor. Its activity is assessed by measuring its ability to scavenge free radicals (e.g., using DPPH or ABTS assays) or to chelate metal ions.
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| Cell Assay |
Cell cycle analysis [5]
Cell Types: PT4 cell Tested Concentrations: 0.7 mM Incubation Duration: 48 h Experimental Results: The percentage of PT4 cells in S phase and G2/M phase increased, and the percentage of cells in G0/G1 phase diminished. . In vitro cellular assays for Sodium catechol sulfate involve treating cells with the compound to assess its protective effects against oxidative stress. Cells are exposed to an oxidative stressor (e.g., hydrogen peroxide) in the presence or absence of the compound, and cell viability, ROS levels, and markers of oxidative damage are measured. |
| Animal Protocol |
Animal/Disease Models: balb/c (Bagg ALBino) mouse challenged with ovalbumin (OVA) aerosol for 8 weeks [2]
Doses: 200 mg/ kg Route of Administration: po (oral gavage). Experimental Results: NOx, IL-13 and TGF-β1 levels and NF-κB immunoreactivity were suppressed. Animal/Disease Models: STZ-induced diabetic mice [3] Doses: 300 mg/kg Route of Administration: intraperitoneal (ip) injection, one time/day for two weeks. Experimental Results: diminished levels of total oxidized proteins and advanced glycation end products (AGE)-related proteins. Inhibit cardiomyocyte apoptosis. PKCδ localization at the plasma membrane is diminished. In vivo animal models for Sodium catechol sulfate are used to study its protective effects against oxidative stress and metal toxicity. The compound is typically administered orally or intraperitoneally to assess its efficacy in models of diseases where oxidative stress plays a role, such as ischemia-reperfusion injury or neurodegenerative diseases. |
| ADME/Pharmacokinetics |
Sodium catechol sulfate has a molecular formula of C6H4Na2O8S2 and a molecular weight of 314.20 g/mol. It is also known as Tiron, Tiferron, and 4,5-Dihydroxy-1,3-benzenedisulfonic acid disodium salt. The compound is a white powder that is very freely soluble in water. It should be stored at room temperature.
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| Toxicity/Toxicokinetics |
Sodium catechol sulfate (Tiron) is considered a non-toxic chelating agent. It has an oral bioactive antioxidant activity. The compound is well-tolerated in animal models at certain doses. However, as with any chemical, appropriate safety precautions should be followed when handling it. It is not intended for human therapeutic use.
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| References |
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| Additional Infomation |
Tiron is an organic molecular entity. It is a colorimetric reagent used to determine complexes of iron, manganese, titanium, molybdenum, and zirconium. (From Merck Index, 11th edition)
Sodium catechol sulfate (CAS# 149-45-1), also known as Tiron, is a non-toxic chelating agent and a cell-penetrating antioxidant that scavenges hydroxyl radicals and superoxide. It is used in research to study oxidative stress and metal toxicity. The compound is for research use only and is not approved for human therapeutic use. |
| Molecular Formula |
C6H4NA2O8S2
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|---|---|
| Molecular Weight |
314.2007
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| Exact Mass |
313.914
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| CAS # |
149-45-1
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| Related CAS # |
149-46-2 (free acid);149-45-1 (sodium);
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| PubChem CID |
9001
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| Appearance |
Off-white to light yellow solid powder
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| Melting Point |
300 °C
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| LogP |
1.003
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
18
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| Complexity |
413
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
ISWQCIVKKSOKNN-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C6H6O8S2.2Na/c7-4-1-3(15(9,10)11)2-5(6(4)8)16(12,13)14;;/h1-2,7-8H,(H,9,10,11)(H,12,13,14);;/q;2*+1/p-2
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| Chemical Name |
disodium;4,5-dihydroxybenzene-1,3-disulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~83.33 mg/mL (~265.21 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.62 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.62 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1827 mL | 15.9134 mL | 31.8269 mL | |
| 5 mM | 0.6365 mL | 3.1827 mL | 6.3654 mL | |
| 10 mM | 0.3183 mL | 1.5913 mL | 3.1827 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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