| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
|
||
| 25g |
|
||
| Other Sizes |
|
| ln Vitro |
Chinese rat hamster V79 cell scaffolds are shielded from H2O2-induced cytotoxicity by titan (10 mM) [1]. In superoxygen scaffolds, tiron (0–20 mM) guards against metal-mediated strand breaking of supercoiled DNA scaffolds [1]. In freshly transplanted cells, titan (50 nM-200 nM, 48 h) lowers osteopontin [3]. In freshly transplanted cells, titan (50 nM-200 nM, 48 h) lowers osteopontin [3]. In HDF, tiron (0.2 mM, 2 hours) suppresses the overexpression of MMP-1 and MMP-3 caused by UVB [4]. The percentage of PT4 cells in S phase and G2/M phase is increased by tiron (0.7 mM, 48 hours) [5].
|
|---|---|
| ln Vivo |
In a mouse airway model, tiron (200 mg/kg, gavage) enhances induction and oxidation [2]. Tiron attenuates STZ induction (300 mg/kg, ip, once daily for one week).
|
| Cell Assay |
Cell cycle analysis [5]
Cell Types: PT4 cell Tested Concentrations: 0.7 mM Incubation Duration: 48 h Experimental Results: The percentage of PT4 cells in S phase and G2/M phase increased, and the percentage of cells in G0/G1 phase diminished. . |
| Animal Protocol |
Animal/Disease Models: balb/c (Bagg ALBino) mouse challenged with ovalbumin (OVA) aerosol for 8 weeks [2]
Doses: 200 mg/ kg Route of Administration: po (oral gavage). Experimental Results: NOx, IL-13 and TGF-β1 levels and NF-κB immunoreactivity were suppressed. Animal/Disease Models: STZ-induced diabetic mice [3] Doses: 300 mg/kg Route of Administration: intraperitoneal (ip) injection, one time/day for two weeks. Experimental Results: diminished levels of total oxidized proteins and advanced glycation end products (AGE)-related proteins. Inhibit cardiomyocyte apoptosis. PKCδ localization at the plasma membrane is diminished. |
| References |
|
| Additional Infomation |
Tiron is an organic molecular entity.
A colorimetric reagent for iron, manganese, titanium, molybdenum, and complexes of zirconium. (From Merck Index, 11th ed) |
| Molecular Formula |
C6H4NA2O8S2
|
|---|---|
| Molecular Weight |
314.2007
|
| Exact Mass |
313.914
|
| CAS # |
149-45-1
|
| Related CAS # |
149-46-2 (free acid);149-45-1 (sodium);
|
| PubChem CID |
9001
|
| Appearance |
Off-white to light yellow solid powder
|
| Melting Point |
300 °C
|
| LogP |
1.003
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
18
|
| Complexity |
413
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
ISWQCIVKKSOKNN-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/C6H6O8S2.2Na/c7-4-1-3(15(9,10)11)2-5(6(4)8)16(12,13)14;;/h1-2,7-8H,(H,9,10,11)(H,12,13,14);;/q;2*+1/p-2
|
| Chemical Name |
disodium;4,5-dihydroxybenzene-1,3-disulfonate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~83.33 mg/mL (~265.21 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.62 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.62 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1827 mL | 15.9134 mL | 31.8269 mL | |
| 5 mM | 0.6365 mL | 3.1827 mL | 6.3654 mL | |
| 10 mM | 0.3183 mL | 1.5913 mL | 3.1827 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
