| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Targets |
SMPT does not possess a specific pharmacological target of its own. Its function is chemical rather than biological—the NHS ester and pyridyldithio groups provide reactive handles for bioconjugation. The compound is used to crosslink proteins or to conjugate drugs to antibodies.
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|---|---|
| ln Vitro |
ADC cytotoxins are connected to antibodies through an ADC connector to form ADCs [1].
As a crosslinking reagent, SMPT does not exhibit intrinsic biological activity. Its activity is evaluated in the context of the conjugates prepared using this reagent. The compound enables the formation of stable conjugates between proteins and other molecules. |
| ln Vivo |
In vivo activity of SMPT is realized through the conjugates prepared using this reagent, such as antibody-drug conjugates.
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| Enzyme Assay |
Characterization involves HPLC, NMR, and MS. Storage: appropriate conditions to maintain stability.
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| Cell Assay |
Cell-based studies are conducted in the context of ADC development. ADCs incorporating SMPT linkers are evaluated in cell lines for binding, internalization, and cytotoxicity.
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| Animal Protocol |
In vivo studies with ADCs incorporating SMPT are performed in mouse xenograft models.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are characterized in the context of the complete ADC.
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| Toxicity/Toxicokinetics |
SMPT is not intended for human or veterinary use as a standalone compound. Standard laboratory safety precautions should be followed.
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| References | |
| Additional Infomation |
SMPT is a heterobifunctional crosslinking reagent used in bioconjugation and ADC development. The compound is not approved for clinical use as a standalone compound.
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| Molecular Formula |
C18H16N2O4S2
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|---|---|
| Molecular Weight |
388.46064
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| Exact Mass |
388.055
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| CAS # |
112241-19-7
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| PubChem CID |
130706
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.43g/cm3
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| Boiling Point |
547ºC at 760mmHg
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| Flash Point |
284.6ºC
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| Vapour Pressure |
5.11E-12mmHg at 25°C
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| Index of Refraction |
1.675
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| LogP |
3.741
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
26
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| Complexity |
521
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
GKSPIZSKQWTXQG-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C18H16N2O4S2/c1-12(25-26-15-4-2-3-11-19-15)13-5-7-14(8-6-13)18(23)24-20-16(21)9-10-17(20)22/h2-8,11-12H,9-10H2,1H3
|
| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 4-[1-(pyridin-2-yldisulfanyl)ethyl]benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~200 mg/mL (~514.85 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (12.87 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (12.87 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5743 mL | 12.8713 mL | 25.7427 mL | |
| 5 mM | 0.5149 mL | 2.5743 mL | 5.1485 mL | |
| 10 mM | 0.2574 mL | 1.2871 mL | 2.5743 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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