| Size | Price | Stock | Qty |
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SMIFH2 (SMIFH 2; SMIFH2) is a novel and potent small molecule inhibitor of formin-mediated actin or formin homology 2 (FH2) domains. It prevents formin-mediated actin nucleation and barbed end elongation, disrupts formin-dependent actin cytoskeletal structures in fission yeast and mammalian NIH 3T3 fibroblasts.
| ln Vitro |
In U2OS cells, SMIFH2 (25 uM; 1–16 hours) causes dynamic cytoskeletal reorganization [1]. Protease-independent, SMIFH2 (25 μM) lowers p300, mDia2, and p53 levels [1]. By influencing cytoskeletal reorganization through a post-transcriptional, proteasome-independent mechanism, SMIFH2 lowers p53 expression and function [1].
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| Cell Assay |
Western Blot Analysis[1]
Cell Types: 293T, A375, U2OS and MDA-MB-231 Cell Tested Concentrations: 25 μM Incubation Duration: 5 hrs (hours) for 293T, U2OS, MDA-MB-231 cells; 2.5 hrs (hours) for A375 cells Experimental Results: mDia2, p53 and p300 protein levels were downregulated. |
| References |
| Molecular Formula |
C15H9N2O3SBR
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|---|---|
| Molecular Weight |
377.21256
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| Exact Mass |
375.952
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| CAS # |
340316-62-3
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| PubChem CID |
2258538
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| Appearance |
Light yellow to green yellow solid powder
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| LogP |
3.214
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
22
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| Complexity |
537
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1/C(/C(NC(=S)N1C2=CC=CC(Br)=C2)=O)=C/C3=CC=CO3
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| InChi Key |
MVFJHEQDISFYIS-XYOKQWHBSA-N
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| InChi Code |
InChI=1S/C15H9BrN2O3S/c16-9-3-1-4-10(7-9)18-14(20)12(13(19)17-15(18)22)8-11-5-2-6-21-11/h1-8H,(H,17,19,22)/b12-8+
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| Chemical Name |
(5E)-1-(3-bromophenyl)-5-(furan-2-ylmethylidene)-2-sulfanylidene-1,3-diazinane-4,6-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~331.38 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.08 mg/mL (5.51 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6510 mL | 13.2552 mL | 26.5104 mL | |
| 5 mM | 0.5302 mL | 2.6510 mL | 5.3021 mL | |
| 10 mM | 0.2651 mL | 1.3255 mL | 2.6510 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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