Size | Price | Stock | Qty |
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250mg |
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500mg |
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1g |
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2g |
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5g |
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Other Sizes |
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Purity: = 99.62%
SMCC, formerly known as succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate, is a heterobifunctional protein crosslinker. SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). SMCC conjugation occurs via a maleimide group that is sulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive and forms stable, covalent protein crosslinks.
ln Vitro |
OVA and keyhole limpet hemocyanin (KLH) can be conjugated using SMCC or sulfo-SMCC. 300 μl KLH or OVA (3 mg) and 30 μl SMCC or Sulfo-SMCC (final concentration of 0.1 mM) are added in PBS with a final volume of 3 ml to the conjugation system. [1]
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ln Vivo |
The SMCC-conjugation technique allows antigen-coupled syngeneic splenic mononuclear cells to be injected into mice, eliciting strong immune responses to whole protein or peptide antigens[1].
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References |
[2]. Li F, Wang Z, Huang Y, Xu H, He L, Deng Yan, Zeng X, He N. Delivery of PUMA Apoptosis Gene Using Polyethyleneimine-SMCC-TAT/DNA Nanoparticles: Biophysical Characterization and In Vitro Transfection Into Malignant Melanoma Cells. J Biomed Nanotechnol. 2015 Oct;11(10):1776-82. PubMed PMID: 26502640.
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Molecular Formula |
C16H18N2O6
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Molecular Weight |
334.328
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Exact Mass |
334.1165
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Elemental Analysis |
C, 57.48; H, 5.43; N, 8.38; O, 28.71
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CAS # |
64987-85-5
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Appearance |
Solid powder
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SMILES |
O(C(C1([H])C([H])([H])C([H])([H])C([H])(C([H])([H])N2C(C([H])=C([H])C2=O)=O)C([H])([H])C1([H])[H])=O)N1C(C([H])([H])C([H])([H])C1=O)=O
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InChi Key |
JJAHTWIKCUJRDK-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C16H18N2O6/c19-12-5-6-13(20)17(12)9-10-1-3-11(4-2-10)16(23)24-18-14(21)7-8-15(18)22/h5-6,10-11H,1-4,7-9H2
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Chemical Name |
N-Succinimidyl 4-(N-Maleimidomethyl)cyclohexanecarboxylate
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Synonyms |
SMCC, heterobifunctional cross-linker SMCC crosslinker
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 25 mg/mL (~74.78 mM )
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9911 mL | 14.9553 mL | 29.9106 mL | |
5 mM | 0.5982 mL | 2.9911 mL | 5.9821 mL | |
10 mM | 0.2991 mL | 1.4955 mL | 2.9911 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.