| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
DNA damage is caused by sepin-1 (0, 20, 40 µM; 24 h), which also prevents cell migration and wound healing [1]. In a simulated varied mode of expression, Sepin-1 (0, 10, 20, 40 µM; 24 h) suppresses the protein levels of Plk1, Cdk1, pericentrin, Aurora, and Lamin B1[1]. Assay for cell proliferation. Lower the amounts of mRNA and the expression of the FoxM1 protein [1].
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| ln Vivo |
In mice, sepin-1 (10 mg/kg; intravenously administered; once daily, five days a week for three weeks) exhibits anti-tumor action [2].
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| Cell Assay |
Cell proliferation assay. Reduce the expression of FoxM1 protein and mRNA levels [1]. [1]
Cell Types: BT-474, MCF7, MDA-MB-231, MDA-MB-468 Cell Tested Concentrations: 0-64 µM Incubation Duration: 3 days Experimental Results: Inhibited cell growth, EC50 was 18.03, 17.66, 27.33, 27.92 µM is applicable to BT-474, MCF7, MDA-MB-231, and MDA-MB-468 cells respectively. Western Blot Analysis[1] Cell Types: BT-474, MCF7, MDA-MB-231, MDA-MB-468 Cell Tested Concentrations: 0, 10, 20, 40 µM Incubation Duration: 24 hrs (hours) Experimental Results: FoxM1 protein expression diminished with dose Decreases the expression of A-Raf, B-Raf and C-Raf in a dependent manner. |
| Animal Protocol |
Animal/Disease Models: SCID beige mice (MCF7 xenograft) [2]
Doses: 10 mg/kg Route of Administration: IV; one time/day, 5 days a week for 3 weeks Experimental Results: Inhibition of tumor growth. |
| References |
| Molecular Formula |
C9H9N3O4
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|---|---|
| Molecular Weight |
223.19
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| Exact Mass |
223.059
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| CAS # |
163126-81-6
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| PubChem CID |
2736269
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| Appearance |
Brown to reddish brown solid powder
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| LogP |
1
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
16
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| Complexity |
338
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
YLVSVJDCTDBERH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H9N3O4/c1-9(2)10(13)7-4-3-6(12(15)16)5-8(7)11(9)14/h3-5H,1-2H3
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| Chemical Name |
2,2-dimethyl-5-nitro-3-oxidobenzimidazol-1-ium 1-oxide
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| Synonyms |
Sepin-1 Sepin1 Sepin 1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~448.05 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4805 mL | 22.4024 mL | 44.8049 mL | |
| 5 mM | 0.8961 mL | 4.4805 mL | 8.9610 mL | |
| 10 mM | 0.4480 mL | 2.2402 mL | 4.4805 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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