| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| ln Vitro |
The maximum inhibitory effect of scopone (0-0.4 nM; 24-48 hours) on PSC proliferation is observed at 0.4 nM [1].
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|---|---|
| ln Vivo |
Rats with DBTC-induced pancreatitis benefit greatly from scopone (oral; 30 mg/kg, 60 mg/kg; 4 weeks) in terms of pancreatic weight loss [1].
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| Animal Protocol |
Animal/Disease Models: Chronic pancreatitis (CP) rat model [1]
Doses: 30 mg/kg, 60 mg/kg Route of Administration: po (po (oral gavage)) 30 mg/kg, 60 mg/kg; 4-week Experimental Results: Prevent pancreatic damage Chronic Pancreatitis (CP) Rat Model and Treatment:** Male Sprague Dawley rats (250-300g) were used. Chronic pancreatitis was induced by a single caudal vein injection of dibutyltin dichloride (DBTC) at a dose of 8 mg/kg. DBTC was dissolved in a mixture of ethanol and glycerol (2:3 ratio) to a final concentration of 8 mg/mL. The control group received the vehicle (ethanol/glycerol mixture). One day after DBTC infusion, rats were randomly assigned to treatment groups. Scoparone was administered orally at doses of 30 mg/kg (low dose) or 60 mg/kg (high dose) in 1.5 mL of saline daily. The salvianolic acid B group received 10 mg/kg orally. The control and CP groups received the same volume of saline. The treatment continued for 4 weeks, after which pancreatic tissue and serum were collected for analysis. [1] - **Histological Examination:** After sacrifice, pancreases were removed, weighed, and processed for histological analysis. Two pathologists scored the tissue samples on six indicators: inflammation, edema, abnormal architecture, glandular atrophy, fibrosis, and pseudotubular complexes. [1] - **Biochemical Parameter Detection:** Serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and creatinine (Cr) were assayed using commercially available kits to assess liver and kidney function. [1] - **Measurement of Pancreatic MDA and SOD:** Pancreatic tissue homogenates were used to quantify the levels of MDA and SOD using commercially available detection kits, following the manufacturer's instructions. [1] - **Western Blotting of Pancreatic Tissue:** The expression of α-SMA, collagen I, TGF-β, Smad2/3, p-Smad2/3, and Smad7 in pancreatic tissue was determined by Western blotting, following similar procedures as described for the cell assays. [1] |
| References | |
| Additional Infomation |
Scoparone belongs to the coumarin class of compounds, and its structure is similar to that of aescin, except that the two hydroxyl groups at positions 6 and 7 are replaced by methoxy groups. It is a major component of the traditional Chinese medicine anemone and possesses various pharmacological activities, such as anti-inflammatory, anti-allergic, and anti-tumor activities. It can be used as a plant metabolite, anti-inflammatory drug, lipid-lowering drug, immunosuppressant, antihypertensive drug, and anti-allergic drug. Scoparone belongs to the coumarin class of aromatic ethers, and its function is similar to that of aescin. Scoparone has been reported to exist in dwarf snow lotus (Saussurea eopygmaea), Cedrelopsis grevei, and other organisms with relevant data.
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| Molecular Formula |
C11H10O4
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|---|---|
| Molecular Weight |
206.1947
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| Exact Mass |
206.057
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| Elemental Analysis |
C, 64.08; H, 4.89; O, 31.04
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| CAS # |
120-08-1
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| PubChem CID |
8417
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| Appearance |
Solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
369.2±42.0 °C at 760 mmHg
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| Melting Point |
145°C
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| Flash Point |
166.8±27.9 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.557
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| LogP |
1.6
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
15
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| Complexity |
274
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O1C(C([H])=C([H])C2=C([H])C(=C(C([H])=C12)OC([H])([H])[H])OC([H])([H])[H])=O
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| InChi Key |
GUAFOGOEJLSQBT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H10O4/c1-13-9-5-7-3-4-11(12)15-8(7)6-10(9)14-2/h3-6H,1-2H3
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| Chemical Name |
6,7-dimethoxychromen-2-one
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| Synonyms |
Escoparone; Scoparone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~242.49 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (10.09 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (10.09 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (10.09 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8499 mL | 24.2495 mL | 48.4990 mL | |
| 5 mM | 0.9700 mL | 4.8499 mL | 9.6998 mL | |
| 10 mM | 0.4850 mL | 2.4249 mL | 4.8499 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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