| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
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| ln Vitro |
The primary metabolite of antibody-maytansinoid conjugates made with the thiol-containing maytansinoid DM1 is S-methylDM1 in the cell or liver [1]. Microtubule assembly is inhibited by S-methylDM1 at a half-maximal dose of 4 μM. Dynamic instability was decreased more by 100 nM S-methyl-DM1 (84%) than by maytansine (45%). 37 high-affinity sites per microtubule (Kd 0.1 μM) are bound by tritiated S-methyl-DM1 [1]. The concentration-dependent curve of S-methylDM1, which inhibits cell growth, was formed like a S in MCF7 cells. S-methylDM1 had a negligible inhibitory effect at 200 pM and a peak inhibitory effect at 3 nM. The potency of S-methyl DM1 (IC50 = 330 pM) is somewhat higher than that of maytansine (IC50 = 710 pM) [2]. S-methylDM1 increased the amount of cells in MCF7 cells to a maximum of 80% in the G2/M phase, while the control group only had 30% of this amount [2].
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| References |
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| Molecular Formula |
C36H50CLN3O10S
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|---|---|
| Molecular Weight |
752.314308643341
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| Exact Mass |
751.29
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| CAS # |
912569-84-7
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| PubChem CID |
89629084
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| Appearance |
White to off-white solid powder
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| LogP |
2.6
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
51
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| Complexity |
1350
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| Defined Atom Stereocenter Count |
8
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| SMILES |
C[C@@]12O[C@H]1[C@@H]([C@@]1([H])OC(=O)N[C@]([C@@]([H])(C=CC=C(C)CC3C=C(OC)C(Cl)=C(C=3)N(C)C(=O)C[C@]2([H])OC(=O)[C@H](C)N(C)C(=O)CCSC)OC)(O)C1)C |c:17,t:15,&1:1,3,4,5,11,12,34,39|
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| InChi Key |
PLYHSTGTQYPYMT-JLZGXKMHSA-N
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| InChi Code |
InChI=1S/C36H50ClN3O10S/c1-20-11-10-12-27(47-8)36(45)19-26(48-34(44)38-36)21(2)32-35(4,50-32)28(49-33(43)22(3)39(5)29(41)13-14-51-9)18-30(42)40(6)24-16-23(15-20)17-25(46-7)31(24)37/h10-12,16-17,21-22,26-28,32,45H,13-15,18-19H2,1-9H3,(H,38,44)/b12-10+,20-11+/t21-,22+,26+,27-,28+,32+,35+,36+/m1/s1
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| Chemical Name |
[(1S,2R,3S,5S,6S,16E,18E,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.110,14.03,5]hexacosa-10,12,14(26),16,18-pentaen-6-yl] (2S)-2-[methyl(3-methylsulfanylpropanoyl)amino]propanoate
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| Synonyms |
Smethyl DM1; S methyl DM1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~132.92 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.32 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3292 mL | 6.6462 mL | 13.2924 mL | |
| 5 mM | 0.2658 mL | 1.3292 mL | 2.6585 mL | |
| 10 mM | 0.1329 mL | 0.6646 mL | 1.3292 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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