Size | Price | Stock | Qty |
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500mg |
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1g |
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5g |
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10g |
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25g |
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Other Sizes |
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Rotenone is a potent botanical insecticide which acts as an inhibitor of mitochondrial electron transport chain complex I. It specifically inhibits NAD-linked substrate oxidation of NADH dehydrogenase with an EC50=10 pmol/mg. It is known that this interference of the electron transport chain occurs from complex I to ubiquinone, similarly to amytal. Studies indicate that Rotenone inhibits mammalian cell proliferation, via suppressing microtubule assembly by binding to tubulin. Rotenone can inhibit the effects of N-methyl-4-phenylpyridinium on mitochondrial swelling and cytochrome c release. Rotenone is an inhibitor of NDUFS.
ln Vitro |
The way that rotenone affects ENS signaling involves the intimate involvement of mitogen-activated polypeptide (MAPK), Toll-like receptors, Wnt, and Ras [2].
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ln Vivo |
Rotenone can be utilized in animal modeling to develop Parkinson's regulation models. Rotenone induces a considerable rise in excitatory neurotransmitters; significant decreases in glutamate and aspartate, as well as excitatory, GABA, glycine, and taurine, are detected in the cerebellum of PD animals [1]. Rotenone (1.5, 2, or 2.5 mg/kg) resulted in increased alpha-synuclein dose needs in the substantia nigra. Furthermore, at dosages of 2 and 2.5 mg/kg, rotenone induced significant decreases in the number of tyrosine kinase-immunoreactive neurons in the substantia nigra and dopamine in the striatum [4].
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References |
[1]. Khadrawy YA, et al. Cerebellar neurochemical and histopathological changes in rat model of Parkinson's disease induced by intrastriatal injection of rotenone. Gen Physiol Biophys. 2016 Nov 30.
[2]. Guan Q, et al. RNA-Seq Expression Analysis of Enteric Neuron Cells with Rotenone Treatment and Prediction of Regulated Pathways. Neurochem Res. 2016 Nov 30. [3]. Kishore Kumar SN, et al. Morinda citrifolia mitigates rotenone-induced striatal neuronal loss in male Sprague-Dawley rats by preventing mitochondrial pathway of intrinsic apoptosis. Redox Rep. 2016 Nov 24:1-12. [4]. Zhang ZN, et al. Subcutaneous rotenone rat model of Parkinson's disease: dose exploration study. Brain Res. 2016 Nov 19. pii: S0006-8993(16)30776-4. [5]. Li N, et al. Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. J Biol Chem. 2003 Mar 7;278(10):8516-25. |
Molecular Formula |
C23H22O6
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Molecular Weight |
394.42
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Exact Mass |
394.14
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Elemental Analysis |
C, 70.04; H, 5.62; O, 24.34
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CAS # |
83-79-4
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Related CAS # |
83-79-4
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Appearance |
Solid powder
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SMILES |
CC(=C)[C@H]1CC2=C(O1)C=CC3=C2O[C@@H]4COC5=CC(=C(C=C5[C@@H]4C3=O)OC)OC
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InChi Key |
JUVIOZPCNVVQFO-HBGVWJBISA-N
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InChi Code |
InChI=1S/C23H22O6/c1-11(2)16-8-14-15(28-16)6-5-12-22(24)21-13-7-18(25-3)19(26-4)9-17(13)27-10-20(21)29-23(12)14/h5-7,9,16,20-21H,1,8,10H2,2-4H3/t16-,20-,21+/m1/s1
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Chemical Name |
(1S,6R,13S)-16,17-dimethoxy-6-prop-1-en-2-yl-2,7,20-trioxapentacyclo[11.8.0.03,11.04,8.014,19]henicosa-3(11),4(8),9,14,16,18-hexaen-12-one
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Synonyms |
Ro-KO; Rotenone; Cube root; Ronone; HSDB 1762; bCube-Pulver
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: ~50 mg/mL (~126.8 mM)
H2O: < 0.1 mg/mL |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.34 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (6.34 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.34 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 2.5 mg/mL (6.34 mM) in 5% DMSO + 95% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.5354 mL | 12.6768 mL | 25.3537 mL | |
5 mM | 0.5071 mL | 2.5354 mL | 5.0707 mL | |
10 mM | 0.2535 mL | 1.2677 mL | 2.5354 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
J Biol Chem . 2003 Mar 7;278(10):8516-25. J Biol Chem . 2003 Mar 7;278(10):8516-25. td> |
Effect of rotenone on ROS production. J Biol Chem . 2003 Mar 7;278(10):8516-25. td> |
Rotenone-induced DNA laddering and effect of caspase inhibitors on rotenone-induced apoptosis. J Biol Chem . 2003 Mar 7;278(10):8516-25. td> |
Inhibition of rotenone-induced DNA breakdown and DNA laddering by antioxidants. J Biol Chem . 2003 Mar 7;278(10):8516-25. td> |
Inhibition of rotenone-induced cytochromec release and caspase 3 activation by antioxidants. J Biol Chem . 2003 Mar 7;278(10):8516-25. td> |
Effect of Mn-SOD overexpression on rotenone-induced ROS production, cytochrome c release, and caspase activation. J Biol Chem . 2003 Mar 7;278(10):8516-25. td> |
Behavioral analysis in rotenone-infused Parkinsonian rats treated with or without MCE/LD in different time periods. Redox Rep . 2017 Nov;22(6):418-429. td> |
Activity of mitochondrial electron transport chain complexes I (a) and IV (b) in the striatum of rotenone-induced Parkinsonian rats treated with or without MCE/LD. Redox Rep . 2017 Nov;22(6):418-429. td> |