| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
For GABAA receptors, Ro15-4513 typically functions as a partial inverse agonist, with the exception of agonists that contain α4 and α6 subunits [3].
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|---|---|
| ln Vivo |
Complete inhibition of the ethanol (1.8 g/kg)-induced decrease in overall locomotor activity and partial inhibition of the ethanol-induced drop in backup force were observed with Ro 15-4513 (ip; 3 mg/kg; 10 min before testing) [2]. In mice lacking the GABAA receptor delta subunit, Ro 15-4513 (ip injection; 3 mg/kg; 15 minutes prior to 1.5 g/kg ethanol administration) reverses ethanol-induced drowsiness [2].
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| Animal Protocol |
Animal/Disease Models: Male C57BL/6J mice [2]
Doses: 3 mg/kg Route of Administration: intraperitoneal (ip) injection; 10 minutes before test Experimental Results: Complete inhibition of ethanol-induced decrease in total locomotor activity and partial decrease in standing. |
| References |
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| Additional Infomation |
8-azido-5-methyl-6-oxo-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylic acid ethyl ester is an organonitrogen heterocyclic compound and an organic heterotricyclic compound.
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| Molecular Formula |
C16H17N7O3
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|---|---|
| Molecular Weight |
355.36
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| Exact Mass |
326.113
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| CAS # |
91917-65-6
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| PubChem CID |
5081
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| Appearance |
Off-white to yellow solid powder
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| LogP |
1.967
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
24
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| Complexity |
563
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
CFSOJZTUTOQNIA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H14N6O3/c1-3-24-15(23)13-12-7-20(2)14(22)10-6-9(18-19-16)4-5-11(10)21(12)8-17-13/h4-6,8H,3,7H2,1-2H3
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| Chemical Name |
ethyl 8-azido-5-methyl-6-oxo-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate
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| Synonyms |
Ro-15-4513; Ro 154513; Ro 15-4513
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~10 mg/mL (~30.65 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1 mg/mL (3.06 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8140 mL | 14.0702 mL | 28.1405 mL | |
| 5 mM | 0.5628 mL | 2.8140 mL | 5.6281 mL | |
| 10 mM | 0.2814 mL | 1.4070 mL | 2.8140 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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