| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Reynoutrin exerts antioxidant effects by targeting free radicals, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals [1]
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| ln Vitro |
Reynoutrin has a pEC50 of 5.37 against the oxidation of fluorescein, indicating strong antioxidant activity [1].
Reynoutrin, isolated from the leaves of Psidium cattleianum, showed dose-dependent DPPH radical scavenging activity; the scavenging rate increased with increasing concentration of Reynoutrin[1] Reynoutrin exhibited ABTS radical scavenging activity in a concentration-dependent manner, with the activity comparable to that of positive control antioxidants (e.g., ascorbic acid)[1] Reynoutrin demonstrated reducing power, as indicated by increased absorbance at 700 nm with increasing compound concentration, reflecting its ability to donate electrons and inhibit oxidation[1] |
| Enzyme Assay |
DPPH radical scavenging assay: Different concentrations of Reynoutrin solution were mixed with DPPH ethanol solution in equal volume, incubated in the dark at room temperature for 30 min; the absorbance was measured at 517 nm, and the radical scavenging rate was calculated using the formula: [(Absorbance of control - Absorbance of sample)/Absorbance of control] × 100%[1]
ABTS radical scavenging assay: ABTS radical cation solution was prepared by reacting ABTS with potassium persulfate and incubating in the dark for 12-16 h; the solution was diluted with ethanol to an absorbance of 0.70 ± 0.02 at 734 nm; different concentrations of Reynoutrin solution were mixed with the diluted ABTS solution, incubated at room temperature for 6 min, and the absorbance was measured at 734 nm to calculate the scavenging rate[1] Reducing power assay: Different concentrations of Reynoutrin solution were mixed with phosphate buffer and potassium ferricyanide solution, incubated at 50°C for 20 min; trichloroacetic acid solution was added to terminate the reaction, and the mixture was centrifuged; the supernatant was mixed with distilled water and ferric chloride solution, and the absorbance was measured at 700 nm (higher absorbance indicates stronger reducing power)[1] |
| References | |
| Additional Infomation |
Quercetin-3-D-xyloside; Reinutrin has been reported in Rosa multiflora, Toxicodendron wallichii and other organisms with relevant data. Reynoutrin is a flavonoid compound isolated from the leaves of Psidium cattleianum (commonly known as strawberry guava) grown in French Polynesia [1]. The antioxidant mechanism of Reynoutrin is mainly related to its ability to provide hydrogen atoms or electrons to neutralize free radicals, thereby inhibiting lipid peroxidation and oxidative damage to biomolecules [1]. Reynoutrin is one of the key flavonoid components in the overall antioxidant capacity of guava leaf extract [1].
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| Molecular Formula |
C20H18O11
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|---|---|
| Molecular Weight |
434.3503
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| Exact Mass |
434.084
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| CAS # |
549-32-6
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| PubChem CID |
5878729
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.9±0.1 g/cm3
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| Boiling Point |
828.1±65.0 °C at 760 mmHg
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| Melting Point |
201-203℃
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| Flash Point |
296.3±27.8 °C
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| Vapour Pressure |
0.0±3.2 mmHg at 25°C
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| Index of Refraction |
1.802
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| LogP |
1.68
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
31
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| Complexity |
711
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
PZZRDJXEMZMZFD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H18O11/c21-8-4-11(24)14-13(5-8)30-18(7-1-2-9(22)10(23)3-7)19(16(14)27)31-20-17(28)15(26)12(25)6-29-20/h1-5,12,15,17,20-26,28H,6H2
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| Chemical Name |
2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3-(3,4,5-trihydroxyoxan-2-yl)oxychromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~150 mg/mL (~345.34 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.76 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.76 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.76 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3023 mL | 11.5115 mL | 23.0229 mL | |
| 5 mM | 0.4605 mL | 2.3023 mL | 4.6046 mL | |
| 10 mM | 0.2302 mL | 1.1511 mL | 2.3023 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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