| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 25mg |
|
||
| 100mg | |||
| 250mg | |||
| Other Sizes |
| Targets |
Amylase secretion activity in human salivary gland cells. [1]
|
|---|---|
| ln Vitro |
Quercetin-7-O-beta-D-glucopyranoside (compound 11) was tested for its ability to modulate amylase secretion in human salivary gland (HSG) cells treated with 40 mM high glucose. All isolated compounds (1–13) increased amylase secretion activity and reversed the reduction caused by high glucose, except compound 3. Quercetin-7-O-beta-D-glucopyranoside exhibited one of the most potent amylase secretion activities, comparable to the positive control (8-epiisopildiol-3-O-β-D-glucopyranoside). No quantitative values (e.g., EC50, fold increase) were provided. [1]
|
| Cell Assay |
The amylase secretion activity of Quercetin-7-O-beta-D-glucopyranoside was evaluated in human salivary gland (HSG) cells. Cells were treated with high glucose (40 mM) to induce a reduction in amylase secretion, mimicking xerostomia-like conditions. After treatment with the test compounds, amylase activity in the culture supernatant was determined by measuring the levels of nitrophenol, a chromogenic product hydrolyzed by α-amylase. This method is described as a simple, direct kinetic assay for salivary α-amylase. Quercetin-7-O-beta-D-glucopyranoside (compound 11) significantly increased amylase secretion, reversing the inhibitory effect of high glucose, and its potency was comparable to that of the positive control 8-epiisopildiol-3-O-β-D-glucopyranoside. [1]
|
| References |
|
| Additional Infomation |
Quercetin 7-O-β-D-glucoside is a quercetin O-glucoside in which a glucosyl residue is linked to the 7-position of quercetin via a β-glycosidic bond. It possesses antioxidant and metabolic activities. It is a β-D-glucoside, monosaccharide derivative, flavonol compound, tetrahydroxyflavone, and quercetin O-glucoside. Quercetin glycosides have been reported in camellia (Camellia sinensis), spring roll (Spiranthes vernalis), and several other organisms with relevant data. See also: Chamomile (partial).
Quercetin-7-O-beta-D-glucopyranoside (quercimeritrin) was isolated from the leaves of Ixeridium dentatum as one of eleven known compounds. The study aimed to develop I. dentatum as a supplement for dry mouth (xerostomia). Previous research from the same group showed that other sesquiterpene lactones from the roots of I. dentatum (e.g., 8-epiisolipidiol-3-O-β-D-glucopyranoside and ixerin F) were useful to prevent and treat xerostomia. The current study extends this investigation to the leaves, and Quercetin-7-O-beta-D-glucopyranoside was identified as one of the active amylase regulators. [1] |
| Molecular Formula |
C21H20O12
|
|---|---|
| Molecular Weight |
464.3763
|
| Exact Mass |
464.095
|
| CAS # |
491-50-9
|
| PubChem CID |
5282160
|
| Appearance |
White to yellow solid
|
| Density |
1.8±0.1 g/cm3
|
| Boiling Point |
859.2±65.0 °C at 760 mmHg
|
| Flash Point |
302.8±27.8 °C
|
| Vapour Pressure |
0.0±0.3 mmHg at 25°C
|
| Index of Refraction |
1.775
|
| LogP |
-0.17
|
| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
12
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
33
|
| Complexity |
758
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
O1[C@]([H])([C@@]([H])([C@]([H])([C@@]([H])([C@@]1([H])C([H])([H])O[H])O[H])O[H])O[H])OC1=C([H])C(=C2C(C(=C(C3C([H])=C([H])C(=C(C=3[H])O[H])O[H])OC2=C1[H])O[H])=O)O[H]
|
| InChi Key |
BBFYUPYFXSSMNV-HMGRVEAOSA-N
|
| InChi Code |
InChI=1S/C21H20O12/c22-6-13-15(26)17(28)19(30)21(33-13)31-8-4-11(25)14-12(5-8)32-20(18(29)16(14)27)7-1-2-9(23)10(24)3-7/h1-5,13,15,17,19,21-26,28-30H,6H2/t13-,15-,17+,19-,21-/m1/s1
|
| Chemical Name |
2-(3,4-dihydroxyphenyl)-3,5-dihydroxy-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one
|
| Synonyms |
Quercimeritrin
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~71.77 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.38 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.38 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1534 mL | 10.7670 mL | 21.5341 mL | |
| 5 mM | 0.4307 mL | 2.1534 mL | 4.3068 mL | |
| 10 mM | 0.2153 mL | 1.0767 mL | 2.1534 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
