| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 25mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg | |||
| Other Sizes |
Purity: ≥98%
| Targets |
Qstatin's primary target is SmcR, the LuxR homologue in Vibrio species. It acts as a potent and selective inhibitor, binding tightly to SmcR and altering its transcriptional regulatory activity.
|
|---|---|
| ln Vitro |
RLU levels in V. vulnificusWT (pBB1) reveal that QStatin (0.001 μM-10 μM; 5 hours) reduces SmcR activity, with an EC50 of 208.9 nM [1]. Proteases and elastase, two SmcR-activated virulence factors in Vibrio vulnificus, exhibit dose-dependent reductions in activity in response to QStatin (5 μM–50 μM; 16 hours) [1]. Cellular SmcR levels are unaffected by QStatin (5 μM-50 μM; 16 hours), suggesting that QStatin has no influence on SmcR expression or stability [1].
In vitro, Qstatin is a potent and selective inhibitor of SmcR with an EC50 of 208.9 nM. It binds tightly to SmcR and changes the flexibility of the protein. It shows pan-QS inhibitor activity in diverse Vibrio species. |
| ln Vivo |
In vivo, Qstatin can be used as a sustainable antivibriosis agent in aquaculture. By inhibiting quorum sensing, it reduces the virulence of Vibrio species in aquatic hosts.
|
| Enzyme Assay |
The in vitro activity of Qstatin is assessed using quorum sensing reporter assays. Vibrio strains carrying a quorum sensing-regulated reporter gene are treated with various concentrations of Qstatin. The inhibition of reporter gene expression is measured to determine the EC50.
|
| Cell Assay |
For cellular assays, Vibrio species are used. Cells are treated with various concentrations of Qstatin, and the inhibition of quorum sensing-regulated phenotypes, such as bioluminescence or virulence factor production, is assessed.
|
| Animal Protocol |
In vivo, Qstatin is typically administered to aquatic organisms, such as fish or shrimp, to prevent Vibrio infections. Its efficacy is assessed by measuring the reduction in disease incidence and mortality.
|
| ADME/Pharmacokinetics |
Qstatin has a molecular weight of 293.16 g/mol and a molecular formula of C7H5BrN2O2S2. It is soluble in DMSO at ≥60 mg/mL. It should be stored at 2-8°C, protected from light, in a dry, sealed container.
|
| Toxicity/Toxicokinetics |
Specific toxicity data for Qstatin is not available in the provided search results. As a compound intended for aquaculture use, its toxicity to non-target organisms would be assessed.
|
| References |
[1]. QStatin, a Selective Inhibitor of Quorum Sensing in Vibrio Species. MBio. 2018 Jan 30;9(1). pii: e02262-17.
|
| Additional Infomation |
Qstatin is a research compound being developed as a sustainable antivibriosis agent for aquaculture. Its mechanism of action involves inhibiting quorum sensing in Vibrio species. It is not a pharmaceutical drug for human use.
|
| Molecular Formula |
C7H5BRN2O2S2
|
|---|---|
| Molecular Weight |
293.160797834396
|
| Exact Mass |
291.897
|
| CAS # |
902688-24-8
|
| Related CAS # |
902688-24-8
|
| PubChem CID |
3237735
|
| Appearance |
Typically exists as solid at room temperature
|
| LogP |
2.5
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
14
|
| Complexity |
303
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CN(N=C1)S(=O)(=O)C2=CC=C(S2)Br
|
| InChi Key |
MLRBBGFHILYDBK-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C7H5BrN2O2S2/c8-6-2-3-7(13-6)14(11,12)10-5-1-4-9-10/h1-5H
|
| Chemical Name |
1-(5-Bromothiophene-2-sulfonyl)-1H-pyrazole
|
| Synonyms |
Qstatin Q-statin Q statin
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~83.33 mg/mL (~284.25 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.10 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.10 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.10 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4111 mL | 17.0555 mL | 34.1111 mL | |
| 5 mM | 0.6822 mL | 3.4111 mL | 6.8222 mL | |
| 10 mM | 0.3411 mL | 1.7056 mL | 3.4111 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
|
|
|
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA
