| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 100mg | |||
| Other Sizes |
| Targets |
Pyrabactin's primary target is the abscisic acid (ABA) receptor, specifically Pyrabactin Resistance 1 (PYR1). It acts as an agonist, mimicking the action of the plant hormone ABA. It promotes interactions between HAB1 and PYR1, PYL1, or PYL3.
|
|---|---|
| ln Vitro |
In vitro, Pyrabactin acts as a seed-selective ABA agonist. It promotes interactions between the ABA receptor PYR1 and its downstream signaling partners.
|
| ln Vivo |
In vivo, Pyrabactin selectively inhibits seed germination. It is used as a synthetic plant growth inhibitor to study ABA signaling and its role in plant development and stress responses.
|
| Enzyme Assay |
The in vitro activity of Pyrabactin is assessed using protein-protein interaction assays. The ABA receptor PYR1 is incubated with its downstream signaling partner HAB1 in the presence of varying concentrations of Pyrabactin. The promotion of the interaction is measured to determine the compound's agonistic activity.
|
| Cell Assay |
For cellular assays, plant cells or protoplasts are used to study the effects of Pyrabactin on ABA signaling. Cells are treated with various concentrations of Pyrabactin, and the expression of ABA-responsive genes is analyzed.
|
| Animal Protocol |
In vivo, Pyrabactin is typically applied to seeds or plants. Its effects on seed germination, seedling growth, and gene expression are assessed.
|
| ADME/Pharmacokinetics |
Pyrabactin has a molecular weight of 377.26 g/mol and a molecular formula of C16H13BrN2O2S. It is soluble in DMSO at 10 mM.
|
| Toxicity/Toxicokinetics |
Specific toxicity data for Pyrabactin is not available in the provided search results. As a plant growth regulator, its toxicity to mammals is not a primary concern. However, standard laboratory safety precautions should be followed.
|
| References | |
| Additional Infomation |
Pyrabactin is a sulfonamide compound formed by the condensation of the sulfonic acid group of 4-bromonaphthalene-1-sulfonic acid with the exoamino group of 2-pyridinemethylamine. It mimics the action of the plant hormone abscisic acid, possessing dual functions as a hormone, plant growth regulator, and abscisic acid receptor agonist. Pyrabactin belongs to the sulfonamide, pyridine, naphthalene, and organobromine compounds classes.
Pyrabactin is a research compound used to study ABA signaling in plants. Its discovery was crucial for identifying the ABA receptors. It is not a pharmaceutical drug and is not intended for human use. |
| Molecular Formula |
C16H13N2O2SBR
|
|---|---|
| Molecular Weight |
377.256
|
| Exact Mass |
375.988
|
| CAS # |
419538-69-5
|
| PubChem CID |
1125790
|
| Appearance |
White to off-white solid
|
| LogP |
4.947
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
22
|
| Complexity |
465
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C(CNS(C2=CC=C(Br)C3=CC=CC=C23)(=O)=O)N=C1
|
| InChi Key |
GJSDYQXOSHKOGX-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C16H13BrN2O2S/c17-15-8-9-16(14-7-2-1-6-13(14)15)22(20,21)19-11-12-5-3-4-10-18-12/h1-10,19H,11H2
|
| Chemical Name |
4-bromo-N-(pyridin-2-ylmethyl)naphthalene-1-sulfonamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~265.1 mM; with ultrasonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.63 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.63 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (6.63 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6507 mL | 13.2535 mL | 26.5069 mL | |
| 5 mM | 0.5301 mL | 2.6507 mL | 5.3014 mL | |
| 10 mM | 0.2651 mL | 1.3253 mL | 2.6507 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
