| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Py-BODIPY-NHS ester does not have a specific biological target in the traditional sense, as it is a fluorescent dye used for labeling rather than a pharmacologically active compound. However, it has been reported to be optimal for MET kinase studies, suggesting potential utility in kinase research. The compound's primary function is to serve as a fluorescent probe for labeling living cells and immobilizing cells. Its ability to penetrate cell membranes allows it to enter cells and specifically stain lipid droplets. The NHS ester group reacts with primary amines to form stable amide bonds, enabling the attachment of the fluorescent dye to proteins and other amine-containing molecules.
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| ln Vitro |
1. Working solution composition for Py-BODIPY NHS ester 1.1: Stock solution preparation Load 1 milligram of Py-BODIPY NHS ester with 382 μL of anhydrous DMSO to extract the 10 mM stock solution. Note: To make the Py-BODIPY NHS ester working solution at 1–10 μM, it is advised to aliquot the Py-BODIPY NHS ester storage solution and swap out the 1.2 working solution with the pre-made serum-free cell culture medium or PBS. ..Note: Please prepare Py-BODIPY NHS ester working solution for use by adjusting its concentration to suit the current circumstances. 2. Suspended cell staining (2.1): Centrifuge cells, add PBS, then wash twice for five minutes each time. For a duration of 5 to 30 minutes, add 1 mL of Py-BODIPY NHS ester working solution after the cell density reaches 1×106/2.2. 2.3 After centrifuging for three to four minutes at 400 g, remove the supernatant. 2.4 After adding PBS, wash the cells twice for five minutes each. 2.5 Use fluorescent cells or flow cytometry to observe the cells after resuspending them in 1 mL of serum-free cells or PBS. 3. Adhesion-based cell staining 3.1 Grow adherent cells on clear glass slides. 3.2 Shake gently to cover the cells fully with 100 μL of the dye working solution from culture 3.3, and leave for 5 to 30 minutes. 3.4 Aspirate the dye working solution, rinse with culture medium two or three times for five minutes each, and use a flow cytometer or fluorescence microscope to monitor.
Py-BODIPY-NHS ester exhibits excellent fluorescence properties in vitro, including strong UV absorption, bright red fluorescence, high extinction coefficients, and high quantum yields. Its fluorescence peak is relatively sharp, and it is stable under various physiological conditions. The compound is cell-permeable and can effectively pass through the cell membrane to enter cells, where it specifically stains lipid droplets with polar lipids. The NHS ester group allows for efficient conjugation to primary amines on proteins, peptides, or small molecules, making it a versatile tool for fluorescence labeling applications. |
| ln Vivo |
In vivo activity data for Py-BODIPY-NHS ester are limited, as the compound is primarily used as a research tool for in vitro labeling applications. Its cell-permeable nature suggests that it may be applicable for in vivo imaging studies, but comprehensive in vivo studies have not been extensively reported. The compound's fluorescence properties, including its bright red emission and photostability, make it a candidate for in vivo imaging applications. However, further research is needed to evaluate its biodistribution, clearance, and safety in animal models.
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| Enzyme Assay |
In vitro enzyme/receptor binding experiments are not typically performed with Py-BODIPY-NHS ester, as it is a fluorescent dye rather than a pharmacologically active compound. However, the NHS ester group allows for covalent conjugation to proteins, peptides, or small molecules containing primary amines, enabling the creation of fluorescently labeled probes. These labeled probes can then be used in binding assays to study protein-protein interactions, receptor-ligand binding, or enzyme activity. The compound's fluorescence properties, including its high quantum yield and photostability, make it suitable for such applications.
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| Cell Assay |
The in vitro cellular assay for Py-BODIPY-NHS ester typically involves labeling cells with the fluorescent dye to visualize cellular structures or proteins. Cells are incubated with the compound in culture medium for a defined period, allowing the dye to penetrate the cell membrane and stain specific cellular compartments, such as lipid droplets. After washing to remove unbound dye, cells are analyzed by fluorescence microscopy or flow cytometry. The NHS ester group can also be used to conjugate the dye to antibodies or other targeting molecules for specific labeling applications. Cytotoxicity is assessed in parallel to ensure that the labeling does not adversely affect cell viability.
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| Animal Protocol |
In vivo animal experiments for Py-BODIPY-NHS ester would typically involve administering the compound to animals for imaging applications. The compound's fluorescence properties, including its bright red emission and photostability, make it suitable for in vivo imaging. However, comprehensive in vivo studies have not been extensively reported. Researchers using this compound for in vivo applications would need to evaluate its biodistribution, clearance, and safety in animal models. The compound's cell-permeable nature and ability to stain lipid droplets may be useful for studying lipid metabolism in vivo.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Py-BODIPY-NHS ester have not been extensively characterized, as the compound is primarily used as a research tool for in vitro labeling applications rather than as a therapeutic agent. The compound's molecular weight (426.18) and chemical properties suggest that it may have moderate bioavailability, but detailed studies on its absorption, distribution, metabolism, and excretion are limited. The NHS ester group is reactive and would be expected to form covalent bonds with amine-containing biomolecules in vivo, which could affect its pharmacokinetic profile.
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| Toxicity/Toxicokinetics |
Toxicological data for Py-BODIPY-NHS ester are limited, as the compound is primarily used as a research tool for labeling applications rather than as a therapeutic agent. In vitro cytotoxicity assays are typically performed to ensure that the compound does not adversely affect cell viability at the concentrations used for labeling. Comprehensive in vivo toxicology studies have not been reported. Researchers handling this compound should follow standard safety protocols for handling fluorescent dyes and NHS ester reagents, including the use of personal protective equipment and working in a well-ventilated area.
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| References | |
| Additional Infomation |
Py-BODIPY-NHS ester is a research reagent that has not entered clinical trials or received regulatory approval for therapeutic use. It is primarily used as a fluorescent probe for labeling living cells, immobilizing cells, and staining lipid droplets. The compound's NHS ester group enables conjugation to primary amines on proteins, peptides, or other biomolecules, making it a versatile tool for bioconjugation and fluorescence labeling applications. Its bright red fluorescence, high photostability, and cell-permeable nature make it valuable for cell biology research.
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| Molecular Formula |
C??H??BF?N?O?
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| Molecular Weight |
426.18
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| Exact Mass |
426.131
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| CAS # |
201998-61-0
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| PubChem CID |
138115341
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| Appearance |
Brown to black solid powder
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
31
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| Complexity |
918
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[F-][B+3]1([N-]2C(=CC=C2C=C2C=CC(C3NC=CC=3)=N12)CCC(=O)ON1C(CCC1=O)=O)[F-]
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| InChi Key |
CRGDXALGAHKHRS-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H17BF2N4O4/c22-21(23)25-13(6-10-20(30)31-27-18(28)8-9-19(27)29)3-4-14(25)12-15-5-7-17(26(15)21)16-2-1-11-24-16/h1-5,7,11-12,24H,6,8-10H2
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 3-[2,2-difluoro-12-(1H-pyrrol-2-yl)-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-4-yl]propanoate
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| Synonyms |
PyBODIPYNHS ester; Py BODIPY NHS ester
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~58.66 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.88 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.88 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3464 mL | 11.7321 mL | 23.4643 mL | |
| 5 mM | 0.4693 mL | 2.3464 mL | 4.6929 mL | |
| 10 mM | 0.2346 mL | 1.1732 mL | 2.3464 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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