| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
PV-10 sodium targets multiple cellular components. It is a potent inhibitor of VGlut (vesicular glutamate transporter) with a Ki of 19 nM and vesicular monoamine transporter (VMAT) with a Ki of 64 nM. It also acts as a photosensitizer, generating reactive oxygen species upon light activation.
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|---|---|
| ln Vitro |
In vitro, PV-10 sodium acts as an inhibitor of VGlut and VMAT. It is also used as a diagnostic dye to stain damaged cells. Its antiviral effects have been observed in vitro.
|
| ln Vivo |
In vivo, PV-10 sodium is used as an ophthalmic diagnostic agent. It is applied topically to the eye to detect dry or damaged cells on the ocular surface. It has also been studied for its potential as a photosensitizer in photodynamic therapy.
|
| Enzyme Assay |
The in vitro activity of PV-10 sodium as a VMAT inhibitor is assessed using radioligand binding assays. Membrane preparations from cells expressing VMAT are incubated with a radiolabeled ligand (e.g., [3H]dihydrotetrabenazine) in the presence of varying concentrations of PV-10 sodium. The displacement of the radiolabeled ligand is measured to determine the Ki.
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| Cell Assay |
For cellular assays, PV-10 sodium is used to stain cells for diagnostic or research purposes. Cells are incubated with the dye, and the uptake or binding is visualized using fluorescence microscopy or flow cytometry.
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| Animal Protocol |
In vivo, PV-10 sodium is typically administered topically to the eye as an ophthalmic diagnostic agent. It is applied as a solution, and the staining pattern is observed using a slit lamp.
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| ADME/Pharmacokinetics |
PV-10 sodium has the molecular formula C20H2Cl4I4Na2O5. It is a deep red powder that is soluble in water. It should be stored under appropriate conditions to maintain its stability.
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| Toxicity/Toxicokinetics |
PV-10 sodium can cause convulsions, respiratory depression, changes in liver weight, other liver changes, and effects on dehydrogenases in acute intravenous studies in mice. It may also cause irritation. As a diagnostic agent, it is generally safe for topical ophthalmic use.
|
| References | |
| Additional Infomation |
See also: Rose Red AT (with active fraction).
PV-10 sodium (Rose Bengal sodium) is a synthetic dye with multiple applications. It is used as an ophthalmic diagnostic agent to detect ocular surface damage. It also has antiviral effects and is used as a research tool to study vesicular transporters. It is not a pharmaceutical drug for systemic use. |
| Molecular Formula |
C20H2CL4I4NA2O5
|
|---|---|
| Molecular Weight |
1017.6363
|
| Exact Mass |
1015.463
|
| CAS # |
632-69-9
|
| PubChem CID |
32343
|
| Appearance |
Brown to reddish brown solid powder
|
| Boiling Point |
757.8ºC at 760 mmHg
|
| Melting Point |
>300°C
|
| Flash Point |
412.1ºC
|
| LogP |
8.104
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
35
|
| Complexity |
936
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
UWBXIFCTIZXXLS-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/C20H4Cl4I4O5.2Na/c21-10-8(9(20(31)32)11(22)13(24)12(10)23)7-3-1-5(25)16(29)14(27)18(3)33-19-4(7)2-6(26)17(30)15(19)28;;/h1-2,29H,(H,31,32);;/q;2*+1/p-2
|
| Chemical Name |
disodium;2,3,4,5-tetrachloro-6-(2,4,5,7-tetraiodo-3-oxido-6-oxoxanthen-9-yl)benzoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~31.25 mg/mL (~30.71 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (24.57 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9827 mL | 4.9133 mL | 9.8267 mL | |
| 5 mM | 0.1965 mL | 0.9827 mL | 1.9653 mL | |
| 10 mM | 0.0983 mL | 0.4913 mL | 0.9827 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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