| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
PTZ-343 does not have a biological target in the traditional sense. It is a chemiluminescence enhancer that acts as an electron transfer mediator in the HRP-catalyzed oxidation of luminol. Its "target" is the HRP enzyme system, where it reacts with HRP-II (the oxidized form of HRP) to regenerate HRP and produce a PTZ-343 radical. This radical then oxidizes luminol anions, leading to light emission.
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| ln Vitro |
In its capacity as an electron transfer mediator, PTZ-343 releases HRP and intensifies (PTZ-343) free radicals when it combines with HRP-II. Luminol anions are quickly oxidized by the enhancer (PTZ-343) free radicals, which causes luminescence [1].
In vitro, PTZ-343 is a potent enhancer of luminol chemiluminescence. It greatly increases the light output of the peroxidase-catalyzed luminol chemiluminescent oxidation reaction by more than 80%. The compound acts as an electron transfer mediator, reacting with HRP-II to release HRP and a PTZ-343 radical, which oxidizes luminol anions to induce light emission. Its activity is characterized by its ability to amplify the chemiluminescent signal in HRP-based assays. |
| ln Vivo |
In vivo, PTZ-343 is not used as a therapeutic agent. It is a research reagent used in chemiluminescence-based assays. It is used in portable chemiluminescence imaging immunoassays for the detection of various analytes, including prostate-specific antigen. Its applications are in diagnostic and analytical research.
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| Enzyme Assay |
The in vitro activity of PTZ-343 is assessed using chemiluminescence assays. HRP is incubated with luminol and hydrogen peroxide in the presence of varying concentrations of PTZ-343. The chemiluminescent signal is measured using a luminometer. The enhancement of the signal is calculated by comparing the light output in the presence and absence of PTZ-343. The concentration of PTZ-343 required for maximal enhancement is determined. The kinetics of the chemiluminescent reaction can also be assessed by measuring the light output over time.
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| Cell Assay |
PTZ-343 is not typically used in cellular assays, as it is a reagent for chemiluminescence detection rather than a modulator of cellular function. However, it can be used in assays where HRP is used as a detection enzyme, such as ELISA or Western blotting. In these assays, PTZ-343 is added to the HRP-substrate mixture to enhance the chemiluminescent signal for detection of the target analyte.
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| Animal Protocol |
PTZ-343 is not administered in animal models as a therapeutic agent. It is used in research applications for the detection of analytes in biological samples. In these applications, the compound is used in vitro to enhance chemiluminescent signals for immunoassays or other detection methods.
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| ADME/Pharmacokinetics |
PTZ-343 has a molecular weight of 343.40 g/mol and a molecular formula of C15H14NNaO3S2. It is a water-soluble compound. It should be stored under appropriate conditions to maintain stability. The compound is a phenothiazine derivative with a sulfonate group that confers water solubility.
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| Toxicity/Toxicokinetics |
Specific toxicity data for PTZ-343 is not available in the provided search results. As a research reagent used at low concentrations in chemiluminescence assays, it is not expected to have significant toxicity. However, standard laboratory safety precautions should be followed when handling the compound.
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| References |
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| Additional Infomation |
PTZ-343 is a research reagent used as a chemiluminescence enhancer in HRP-based detection systems. It is used in various applications, including ELISA, Western blotting, and immunoassays, to increase the sensitivity of detection. The compound's ability to enhance chemiluminescent signals by more than 80% makes it a valuable tool for sensitive analyte detection. PTZ-343 is not approved for clinical use and is intended for research purposes only. It is available from chemical suppliers for research applications.
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| Molecular Formula |
C15H14NNAO3S2
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|---|---|
| Molecular Weight |
343.3963
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| Exact Mass |
343.031
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| CAS # |
101199-38-6
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| Related CAS # |
101199-38-6 (sodium);129808-92-0 (free acid);
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| PubChem CID |
23689088
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| Appearance |
White to off-white solid powder
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| LogP |
4.37
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
22
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| Complexity |
432
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VKGYKXFNSKEHED-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C15H15NO3S2.Na/c17-21(18,19)11-5-10-16-12-6-1-3-8-14(12)20-15-9-4-2-7-13(15)16;/h1-4,6-9H,5,10-11H2,(H,17,18,19);/q;+1/p-1
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| Chemical Name |
sodium;3-phenothiazin-10-ylpropane-1-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL (~364.01 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (145.60 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9121 mL | 14.5603 mL | 29.1206 mL | |
| 5 mM | 0.5824 mL | 2.9121 mL | 5.8241 mL | |
| 10 mM | 0.2912 mL | 1.4560 mL | 2.9121 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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