| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| 1g |
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| 2g |
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| 5g |
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| Other Sizes |
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| ln Vitro |
Protonacladehyde (PCA) treatment of MCF-7 cells at 50, 100 μM for 24 and 48 hours significantly decreased cell proliferation by 11% and 20% in the 24 hour period and by 22% and 27% in the 48 hour period, respectively. [2]. MCF-7 cells treated with 50 μM and 100 μM of protocatechuic aldehyde for 24 hours showed a 1.9- and 2.6-fold increase, respectively. PCA suppresses the enzymatic activity of HDAC and inhibits estrogen receptor (ER) active (MCF-7) mammary gland protocatechualdehyde (0, 100, and 200 μM, in HCT116 and SW480 cells, for 48 hours).
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| Cell Assay |
Cell proliferation assay [2]
Cell Types: Human breast cancer cells (MCF-7 and MDA-MB-231) Tested Concentrations: 0, 5, 10, 25, PCA treatment was observed to inhibit HDAC activity in a dose-dependent manner [3]. , 50 and 100 μM Incubation Duration: 24, 48 hrs (hours) Experimental Results: Inhibition of MCF-7 cell growth. Apoptosis analysis [2] Cell Types: Human breast cancer cells (MCF-7 and MDA-MB-231) Tested Concentrations: 0, 5, 10, 25, 50 and 100 μM Incubation Duration: 24, 48 hrs (hours) Experimental Results: Apoptosis Increased apoptosis of MCF-7 cells. |
| References |
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| Additional Infomation |
3,4-dihydroxybenzaldehyde is a dihydroxybenzaldehyde.
Also known as protocatechuic aldehyde, protocatechualdehyde is a naturally-occuring phenolic aldehyde that is found in barley, green cavendish bananas, grapevine leaves and root of the herb S. miltiorrhiza. Protocatechualdehyde possesses antiproliferative and pro-apoptotic properties against human breast cancer cells and colorectal cancer cells by reducing the expression of pro-oncogenes β-catenin and cyclin D1. 3,4-Dihydroxybenzaldehyde has been reported in Salvia miltiorrhiza, Hymenochaete xerantica, and other organisms with data available. See also: Black Cohosh (part of). |
| Molecular Formula |
C7H6O3
|
|---|---|
| Molecular Weight |
138.1207
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| Exact Mass |
138.031
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| CAS # |
139-85-5
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| PubChem CID |
8768
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| Appearance |
Light yellow to brown solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
295.4±20.0 °C at 760 mmHg
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| Melting Point |
150-157 °C(lit.)
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| Flash Point |
146.7±18.3 °C
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| Vapour Pressure |
0.0±0.6 mmHg at 25°C
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| Index of Refraction |
1.674
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| LogP |
1.14
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| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
10
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| Complexity |
124
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
IBGBGRVKPALMCQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C7H6O3/c8-4-5-1-2-6(9)7(10)3-5/h1-4,9-10H
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| Chemical Name |
3,4-dihydroxybenzaldehyde
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 50 mg/mL (~362.00 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (18.10 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (18.10 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (18.10 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.2401 mL | 36.2004 mL | 72.4008 mL | |
| 5 mM | 1.4480 mL | 7.2401 mL | 14.4802 mL | |
| 10 mM | 0.7240 mL | 3.6200 mL | 7.2401 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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