| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Proteinase targets proteins, specifically the peptide bonds that link amino acids together. It catalyzes the hydrolysis of these bonds, breaking down proteins into smaller polypeptides and amino acids. This activity is essential for numerous biological processes, including protein turnover, digestion, and cellular signaling.
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|---|---|
| ln Vitro |
In vitro, Proteinase is characterized by its proteolytic activity. It is used to degrade proteins, to study proteinase inhibitors, and to study thermal inactivation kinetics. It is also used in nucleic acid isolation procedures to remove proteins from DNA and RNA preparations. Its activity is measured by its ability to hydrolyze a protein substrate, such as casein.
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| ln Vivo |
In vivo, Proteinase is not used as a therapeutic agent. However, it plays a crucial role in various physiological processes. It is involved in the digestion of proteins in the gastrointestinal tract. It is also involved in the turnover of cellular proteins and in the regulation of various signaling pathways.
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| Enzyme Assay |
The in vitro enzyme activity assay for Proteinase involves incubating the enzyme with a protein substrate, such as casein or hemoglobin, in a suitable buffer at an optimal pH and temperature. After incubation, the reaction is stopped, and the amount of undigested protein or released amino acids is measured using a colorimetric method, such as the Folin-Ciocalteu assay. One unit of activity is defined as the amount of enzyme that releases 1 μmol of tyrosine per minute under the assay conditions.
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| Cell Assay |
In vitro cell culture studies are not typically performed with Proteinase, as it is a hydrolytic enzyme. However, its effects on protein degradation can be studied in cell lysates. It is also used to detach adherent cells from culture dishes by digesting the extracellular matrix proteins. Its primary use is in biochemical assays and industrial applications.
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| Animal Protocol |
In vivo animal experiments for Proteinase are not common. However, its role in digestion can be studied in animal models of protein metabolism. Its effects on inflammation can be studied in models of pancreatitis. It is not typically administered as a therapeutic agent in animal studies.
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| ADME/Pharmacokinetics |
Proteinase is a protein with a molecular weight that varies depending on its source. It is typically supplied as a lyophilized powder or in aqueous solution. It is stable at room temperature for short periods but is best stored at 4°C or -20°C for long-term stability. Its activity is optimal at pH 3.0-7.0, depending on the source.
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| Toxicity/Toxicokinetics |
Toxicological data for Proteinase indicate that it is generally safe for its intended uses. However, inhalation of the enzyme dust may cause respiratory sensitization in susceptible individuals. It is not intended for human therapeutic use. Standard laboratory safety precautions should be followed when handling the enzyme.
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| Additional Infomation |
Proteinase is a research compound and an industrial enzyme with no clinical approval. It is widely used in the food and beverage industry (e.g., for protein hydrolysis), in the leather industry (e.g., for bating), and in the production of protein hydrolysates. It is also used as a research tool for studying protein structure and function and for protein degradation studies.
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| Molecular Formula |
C18N4OCLH15
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|---|---|
| Molecular Weight |
338.7909
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| CAS # |
9001-92-7
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| Appearance |
Light yellow to khaki solid powder
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| SMILES |
[Proteinase]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~50 mg/mL
DMSO : ~20 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9517 mL | 14.7584 mL | 29.5168 mL | |
| 5 mM | 0.5903 mL | 2.9517 mL | 5.9034 mL | |
| 10 mM | 0.2952 mL | 1.4758 mL | 2.9517 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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