| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Propargyl-PEG2-acid is a linker molecule and does not have a specific biological target. Its functional groups are its reactive sites: the propargyl group reacts with azides in copper-catalyzed azide-alkyne cycloaddition (CuAAC); the carboxylic acid group can be activated and reacted with primary amines to form amide bonds.
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|---|---|
| ln Vitro |
ADC cytotoxins are connected to antibodies through an ADC connector to form ADCs [1]. Two distinct ligands, one for the E3 ubiquitin ligase and the other for the target protein, are present in PROTAC and are joined by a linker. PROTAC selectively degrades target proteins by means of the intracellular ubiquitin-proteasome system [1].
The in vitro activity of Propargyl-PEG2-acid is defined by its chemical reactivity. The propargyl group enables efficient CuAAC reactions with azide-containing molecules, and the carboxylic acid group can be activated for amide bond formation. The compound's utility as a linker is assessed by its ability to form stable conjugates. |
| ln Vivo |
Propargyl-PEG2-acid is not a therapeutic agent and is not administered in vivo. It is used as a research tool for the synthesis of bioconjugates, including fluorescent probes, drug conjugates, and affinity reagents.
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| Enzyme Assay |
The reactivity of Propargyl-PEG2-acid is assessed using chemical conjugation assays. The propargyl group is reacted with an azide-containing compound under CuAAC conditions, and the formation of the triazole product is confirmed by analytical methods. The carboxylic acid group is activated with a coupling reagent (e.g., EDC or HATU) and reacted with a primary amine, and the formation of the amide product is confirmed.
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| Cell Assay |
Propargyl-PEG2-acid is used in the synthesis of bioconjugates for cellular assays. For example, it can be used to conjugate a fluorescent dye to a targeting ligand via the carboxylic acid group, or to introduce a propargyl handle for subsequent click labeling. The resulting conjugates can be used for cell imaging, flow cytometry, or other cellular assays.
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| Animal Protocol |
Propargyl-PEG2-acid is not administered in animal models. It is used to synthesize conjugates that are subsequently tested in vivo. The linker's properties, such as solubility and stability, are evaluated through the performance of the conjugate.
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| ADME/Pharmacokinetics |
Propargyl-PEG2-acid has a molecular weight of 172.18 g/mol and a molecular formula of C8H12O4. It is a small, hydrophilic molecule that is soluble in water and organic solvents. The compound should be stored at -20°C under appropriate conditions to maintain stability.
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| Toxicity/Toxicokinetics |
Propargyl-PEG2-acid is a chemical reagent and is not intended for human use. Standard laboratory safety precautions should be followed when handling the compound. Toxicity data for the compound itself is not available.
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| References | |
| Additional Infomation |
Propargyl-PEG2-acid is a research-grade PEG linker used in the synthesis of bioconjugates. The propargyl group enables click chemistry conjugation, and the carboxylic acid group allows for amide bond formation. The PEG2 spacer provides solubility and flexibility. The compound is not approved for clinical use and is intended for research purposes only.
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| Molecular Formula |
C8H12O4
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|---|---|
| Molecular Weight |
172.178483009338
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| Exact Mass |
172.073
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| CAS # |
1859379-85-3
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| PubChem CID |
88878522
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| Appearance |
Colorless to light yellow liquid
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| LogP |
-0.5
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
12
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| Complexity |
167
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
NNWHATPXNWOQKD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H12O4/c1-2-4-11-6-7-12-5-3-8(9)10/h1H,3-7H2,(H,9,10)
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| Chemical Name |
3-(2-prop-2-ynoxyethoxy)propanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~1451.97 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (12.08 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (12.08 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (12.08 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.8079 mL | 29.0394 mL | 58.0788 mL | |
| 5 mM | 1.1616 mL | 5.8079 mL | 11.6158 mL | |
| 10 mM | 0.5808 mL | 2.9039 mL | 5.8079 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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