| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| ln Vitro |
CDK2/MDM2-IN-1 (100, 200 nM, 4 h) inhibits tumor cell proliferation through CDK2/p-Rb and MDM2/p53 pathways [1].
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|---|---|
| ln Vivo |
CDK2/MDM2-IN-1 (25, 50 mg/kg, intraperitoneal injection, for 14 consecutive days) can significantly inhibit the proliferation of mouse colon tumor cells without affecting the mouse body weight [1].
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| Cell Assay |
Western Blot Analysis[1]
Cell Types: HCT116, OVCAR3 Concentration: 100, 200 nM Incubation Duration: 4 h Experimental Results: Reduced the expression of p-Rb, downstream of CDK2. Inhibited MDM2 protein expression and elevated p53 expression. |
| Animal Protocol |
Animal/Disease Models:A nude mouse model of HCT116 transplanted tumor[1]
Doses: 25, 50 mg/kg Route of Administration: i.p. for 14 days Experimental Results: Reduced tumor volume and weight. |
| References |
| Molecular Formula |
C26H33N5O4
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|---|---|
| Molecular Weight |
479.57
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| Appearance |
White to off-white solid
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| SMILES |
[H][C@]1(C2=CC(NC(CC3=CC=C(N4CCCC4=O)C=C3)=O)=NN2)CC[C@@H](OC(N5CCC5(C)C)=O)C1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~208.5 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.21 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.21 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0852 mL | 10.4260 mL | 20.8520 mL | |
| 5 mM | 0.4170 mL | 2.0852 mL | 4.1704 mL | |
| 10 mM | 0.2085 mL | 1.0426 mL | 2.0852 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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