| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
Dimethyl pimelimidate dihydrochloride does not target a specific biological receptor but rather reacts chemically with primary amines (lysine residues) on proteins. Its reactive imidate groups form covalent amidine bonds with amino groups, enabling cross-linking of proteins or immobilization of antibodies onto solid supports such as Protein A or Protein G-agarose. This chemical reactivity makes it a valuable tool in protein biochemistry and affinity purification.
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|---|---|
| ln Vitro |
In vitro, DMP is used to cross-link proteins in solution or to immobilize antibodies onto chromatography resins. The reaction proceeds under mild conditions at pH 7.0-10.0, forming stable amidine bonds. In ChIP assays, DMP enhances signal by cross-linking proteins to DNA, stabilizing the chromatin complexes. The compound's bifunctional nature allows it to bridge two amino groups, creating covalent linkages between adjacent proteins or between proteins and supporting matrices.
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| ln Vivo |
In vivo activity is not applicable for Dimethyl pimelimidate dihydrochloride, as it is a chemical cross-linking reagent used exclusively for in vitro biochemical applications. The compound is not designed or intended for therapeutic use in living organisms. Its reactive imidate groups would rapidly react with biological amines in vivo, leading to non-specific protein cross-linking and potential toxicity, making it unsuitable for systemic administration.
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| Enzyme Assay |
Non-cellular binding assays for DMP involve incubating the compound with protein solutions or affinity matrices under controlled pH conditions. For antibody affinity column preparation, the antibody is first incubated with Protein A or Protein G-agarose, and then DMP is added to covalently couple the antibody to the support via amidine bond formation. The reaction is typically carried out at pH 8-9 and quenched with ethanolamine or Tris buffer to block remaining reactive imidate groups.
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| Cell Assay |
In vitro cell-based experiments using DMP are limited to specialized applications such as ChIP assays, where the compound is added to cell lysates to cross-link proteins to DNA. Cells are treated with DMP, and the cross-linking reaction is allowed to proceed for a specified time before quenching. The cross-linked chromatin is then sheared and immunoprecipitated with target-specific antibodies. The enhanced signal from DMP cross-linking improves the detection of protein-DNA interactions.
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| Animal Protocol |
In vivo animal experiments are not conducted with Dimethyl pimelimidate dihydrochloride, as the compound is a chemical reagent for in vitro protein cross-linking and affinity purification. Its reactive nature and lack of therapeutic relevance preclude its use in animal models. Researchers should focus on its established applications in protein biochemistry and molecular biology techniques.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not relevant for Dimethyl pimelimidate dihydrochloride as it is not a therapeutic compound. The molecular formula is C9H18N2O2·2HCl with a molecular weight of 259.17. It appears as a white to off-white crystalline powder with a melting point of 122°C. The compound is slightly soluble in water and is moisture-sensitive and heat-sensitive. It should be stored refrigerated (0-10°C).
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| Toxicity/Toxicokinetics |
Toxicity information for Dimethyl pimelimidate dihydrochloride indicates that it is irritating to the eyes and skin. The compound is classified as a hazardous chemical (NFPA 704: Health 1, Flammability 1). Appropriate personal protective equipment should be worn when handling this compound. The flash point is not determined. Researchers should consult the safety data sheet (SDS) for detailed hazard information and first aid measures.
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| References | |
| Additional Infomation |
DMP is widely used in protein biochemistry and molecular biology research. It is also known as pimelimidic acid dimethyl ester dihydrochloride or dimethyl heptane-1,7-diimidate dihydrochloride. The CAS number is 58537-94-3 and the PubChem CID is 11402688. The compound's SMILES is COC(=N)CCCCCC(=N)OC.Cl.Cl. It is commonly used to prepare antibody affinity columns and in ChIP assays.
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| Molecular Formula |
C9H20CL2N2O2
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|---|---|
| Molecular Weight |
259.17
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| Exact Mass |
258.09
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| CAS # |
58537-94-3
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| PubChem CID |
11402688
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| Appearance |
White to off-white solid powder
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| Boiling Point |
301.9ºC at 760mmHg
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| Melting Point |
~122 °C (dec.)
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| Flash Point |
136.4ºC
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| LogP |
3.987
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
15
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| Complexity |
151
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| Defined Atom Stereocenter Count |
0
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| SMILES |
Cl[H].Cl[H].O(C([H])([H])[H])/C(/C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])/C(=N/[H])/OC([H])([H])[H])=N\[H]
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| InChi Key |
LRHXBHUTQWIZTN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H18N2O2.2ClH/c1-12-8(10)6-4-3-5-7-9(11)13-2;;/h10-11H,3-7H2,1-2H3;2*1H
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| Chemical Name |
dimethyl heptanediimidate;dihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~100 mg/mL (~385.85 mM; with sonication)
DMSO :~100 mg/mL (385.85 mM; with sonication) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.65 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one),clear solution.
For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 400 μL PEG300 and mix well. Then add 50 μL Tween-80 to the above system and mix well. Then continue to add 450 μL of physiological saline to make up to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.65 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 900 μL of 20% SBE-β-CD saline solution and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8585 mL | 19.2924 mL | 38.5847 mL | |
| 5 mM | 0.7717 mL | 3.8585 mL | 7.7169 mL | |
| 10 mM | 0.3858 mL | 1.9292 mL | 3.8585 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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