| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
IC50: 0.5 μM (sFRP-1)[1]
|
|---|---|
| ln Vitro |
WAY-316606 has an EC50 of 0.65 μM for Wnt-luciferase activity in U2-OS cells[1]. WAY-316606 binds to the secreted frizzled-related protein (sFRP)-1 inhibitor with a KD of 0.08 μM and an EC50 of 0.65 μM for inhibiting sFRP-1. WAY-316606 also binds to sFRP-2, although the intensity is more than 10-fold weaker than the KD of 1 μM. Using a fluorescence polarization binding assay using a fluorescent probe compound and purified human sFRP-1 protein in a competitive binding format, the IC50 of WAY-316606 is 0.5 μM[2].
|
| ln Vivo |
WAY-316606 increases bone formation when tested in the neonatal mouse calvaria assay. WAY-316606 increases total bone area by up to 60% in a dose-dependent manner with an EC50 of approximately 1 nM. WAY-316606 has good water solubility, moderate to low inhibitory activity against cytochrome p450 isozymes (3A4, 2D6, 2C9), and good stability in rat and human liver microsomes (t1/2>60 minutes in each species)[1]. WAY-316606 exhibits a high plasma clearance (77 mL/min/kg, greater than hepatic blood flow) in female Sprague-Dawley rats following a single intravenous bolus dose (2 mg/kg), which results in a rapid decline in drug exposure in plasma regardless of route of administration[2].
|
| References |
| Molecular Formula |
C18H20CLF3N2O4S2
|
|---|---|
| Molecular Weight |
484.94
|
| Exact Mass |
484.05
|
| CAS # |
1781835-02-6
|
| Related CAS # |
WAY 316606; 915759-45-4
|
| PubChem CID |
25186563
|
| Appearance |
White to off-white solid
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
30
|
| Complexity |
747
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1CNCCC1NS(=O)(=O)C2=C(C=CC(=C2)S(=O)(=O)C3=CC=CC=C3)C(F)(F)F.Cl
|
| InChi Key |
YLOYHLUHXQJNGO-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C18H19F3N2O4S2.ClH/c19-18(20,21)16-7-6-15(28(24,25)14-4-2-1-3-5-14)12-17(16)29(26,27)23-13-8-10-22-11-9-13;/h1-7,12-13,22-23H,8-11H2;1H
|
| Chemical Name |
5-(benzenesulfonyl)-N-piperidin-4-yl-2-(trifluoromethyl)benzenesulfonamide;hydrochloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
Typically soluble in DMSO (e.g. 10 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.16 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0621 mL | 10.3106 mL | 20.6211 mL | |
| 5 mM | 0.4124 mL | 2.0621 mL | 4.1242 mL | |
| 10 mM | 0.2062 mL | 1.0311 mL | 2.0621 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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