| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
2'-Deoxyguanosine 5'-monophosphate (dGMP) is a substrate for guanylate kinases, which convert it to dGDP. dGDP is then further phosphorylated to dGTP, a nucleotide precursor used in DNA synthesis. It is also a substrate for 5'-nucleotidase and other nucleotide-metabolizing enzymes. It targets enzymes in the nucleotide salvage pathway.
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| ln Vitro |
In vitro, 5'-dGMP is used as a substrate in DNA polymerase assays to measure the rate of DNA synthesis and to study nucleotide selectivity and fidelity. It is also used to study feedback inhibition of ribonucleotide reductase and as a reference standard for nucleotide analysis by HPLC and LC-MS.
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| ln Vivo |
In animal studies, 5'-dGMP disodium is administered to mice to study the pharmacokinetics of exogenous nucleotides and to investigate their role in immune function and gut health. It is also used to study the nucleotide salvage pathway in vivo.
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| Enzyme Assay |
A non-cell assay using purified guanylate kinase (GMPK) is performed: 5'-dGMP is incubated with ATP in the presence of recombinant GMPK. The production of dGDP is measured by coupling to pyruvate kinase/lactate dehydrogenase (PK/LDH) to monitor NADH oxidation at 340 nm, or by HPLC with UV detection at 254 nm. Alternatively, a radiolabeled [3H]-dGMP substrate can be used.
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| Cell Assay |
Human cancer cell lines (e.g., HeLa, MCF-7) are cultured in media containing 5'-dGMP (10-100 uM) to study nucleotide metabolism and drug resistance. It can be used to rescue cells treated with deoxynucleoside kinase inhibitors or to study the effects of exogenous nucleotides on cell proliferation and DNA repair. Cellular dGTP levels are measured by LC-MS.
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| Animal Protocol |
In animal studies, 5'-dGMP disodium is typically dissolved in saline and administered to mice by intraperitoneal injection (10-50 mg/kg) or oral gavage. Blood and tissue samples (liver, spleen, gut) are collected. Nucleotide levels in plasma and tissues are analyzed by LC-MS/MS. Immune parameters (e.g., T cell proliferation, cytokine production) are measured to assess the immunomodulatory effects of the nucleotide.
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| ADME/Pharmacokinetics |
5'-dGMP is water-soluble (the disodium salt enhances solubility). It is rapidly dephosphorylated in the gut by intestinal alkaline phosphatases and nucleotidases to deoxyguanosine, which is then absorbed. It has a short half-life in the bloodstream (minutes) due to rapid clearance by the kidneys and metabolism by nucleotidases and phosphatases. Systemic bioavailability after oral administration is low (<10%).
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| Toxicity/Toxicokinetics |
The compound is non-toxic at physiological concentrations. High doses may cause mild gastrointestinal distress. In theory, high doses could lead to hyperuricemia due to the metabolism of guanine to uric acid via guanase and xanthine oxidase, but this is not typically observed at research doses. LD₅0 is not established, but the compound is well-tolerated at 50 mg/kg in mice.
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| References | |
| Additional Infomation |
2'-Deoxyguanosine 5'-monophosphate disodium is used as a standard for the analysis of nucleotides by HPLC and LC-MS in clinical diagnostics and research. It is a research tool for studying DNA replication, DNA repair, and purine nucleotide metabolism. It is also used as a supplement to support immune function. It is not an approved drug but is available as a research reagent.
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| Molecular Formula |
C10H12N5NA2O7P
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|---|---|
| Molecular Weight |
391.18
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| Exact Mass |
391.026
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| CAS # |
33430-61-4
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| Related CAS # |
2'-Deoxyguanosine 5'-monophosphate-13C10,15N5 disodium
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| PubChem CID |
135742695
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| Appearance |
White to off-white solid powder
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| Density |
2.32g/cm3
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| Boiling Point |
844.2ºC at 760mmHg
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| Melting Point |
>245°C (dec.)
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| Flash Point |
464.4ºC
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
25
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| Complexity |
556
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1[C@@H]([C@H](O[C@H]1N2C=NC3=C2N=C(NC3=O)N)COP(=O)([O-])[O-])O.[Na+].[Na+]
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| InChi Key |
CTPAMSRBXKGZCJ-FVALZTRZSA-L
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| InChi Code |
InChI=1S/C10H14N5O7P.2Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(22-6)2-21-23(18,19)20;;/h3-6,16H,1-2H2,(H2,18,19,20)(H3,11,13,14,17);;/q;2*+1/p-2/t4-,5+,6+;;/m0../s1
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| Chemical Name |
disodium;[(2R,3S,5R)-5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methyl phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~125 mg/mL (~319.55 mM)
DMSO :< 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (255.64 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5564 mL | 12.7818 mL | 25.5637 mL | |
| 5 mM | 0.5113 mL | 2.5564 mL | 5.1127 mL | |
| 10 mM | 0.2556 mL | 1.2782 mL | 2.5564 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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