| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| Other Sizes |
|
Purity: ≥98%
| Targets |
Endogenous metabolite.
|
|---|---|
| ln Vitro |
Phosphoketolase activity assay [2]
Phosphoketolase activity was measured using the ferric hydroxamate method, based on the chemical conversion of enzymatically produced acetyl-phosphate into ferric acetyl hydroxamate, according to the protocol from. The standard reactions were carried out in 1.5 mL of Eppendorf tube in a total assay volume of 100 μL consisting of 50 mM Tris (pH 7.5), 5 mM MgCl2, 5 mM potassium phosphate, 1 mM thiamine pyrophosphate and 10 mM F6P as a substrate. The crude cell-free extract was added to start the reaction and incubated at 30 °C for 1 h. To stop the enzymatic reaction, 60 μL hydroxylamine hydrochloride (2 M, pH 6.5) was added to 40 µL of assay solution. After incubation for 10 min at room temperature, 120 μL colouring reagent consisting of 15% (w/v) trichloroacetic acid, 4 M HCl, and FeCl3·6H2O (5% [w/v] in 0.1 M HCl) were added to generate ferric hydroxamate, which was then spectrophotometrically quantified at 505 nm by comparing to a series of lithium potassium acetyl-phosphate standards. |
| Enzyme Assay |
Phosphorylation of LiaR by acetyl phosphate
We used acetyl phosphate to investigate the phosphorylation of LiaR by small molecule phosphate donors. Briefly, LiaR (30 μM) in the PB buffer was incubated with lithium potassium acetyl-phosphate (50 mM). The reaction mixture was incubated at 37 °C at different time intervals. The extent of phosphorylation was investigated by 15% SDS-PAGE, containing acrylamide-pedant Phos-tag™AAL-107 at 50 μM. These experiments were repeated two times.
|
| References |
[1]. Low phosphatase activity of LiaS and strong LiaR-DNA affinity explain the unusual LiaS to LiaR in vivo stoichiometry. BMC Microbiol. 2020 Apr 29;20:104.
[2]. Increasing cellular fitness and product yields in Pseudomonas putida through an engineered phosphoketolase shunt. Microb Cell Fact. 2023 Jan 19;22:14. |
| Molecular Formula |
C2H3KLIO5P
|
|---|---|
| Molecular Weight |
184.06
|
| Exact Mass |
183.952
|
| CAS # |
94249-01-1
|
| PubChem CID |
6098323
|
| Appearance |
White to off-white solid powder
|
| Melting Point |
>300ºC(lit.)
|
| LogP |
0.518
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
10
|
| Complexity |
122
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
RLQMPLKXFIXRCV-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/C2H5O5P.K.Li/c1-2(3)7-8(4,5)6;;/h1H3,(H2,4,5,6);;/q;2*+1/p-2
|
| Chemical Name |
lithium;potassium;acetyl phosphate
|
| Synonyms |
94249-01-1; Lithium potassium acetyl phosphate; Acetyl phosphate lithium potassium salt; Acetyl phosphate(lithium potassium); lithium;potassium;acetyl phosphate; SCHEMBL7214154; DTXSID50915877; Acetyl phosphate lithium potassium;
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O :~50 mg/mL (~271.65 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (543.30 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4330 mL | 27.1651 mL | 54.3301 mL | |
| 5 mM | 1.0866 mL | 5.4330 mL | 10.8660 mL | |
| 10 mM | 0.5433 mL | 2.7165 mL | 5.4330 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
COA