| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
Purine targets enzymes involved in nucleotide metabolism, including purine nucleoside phosphorylase (PNP), xanthine oxidase (XO), and hypoxanthine-guanine phosphoribosyltransferase (HGPRT). It is a substrate for these enzymes in the purine salvage pathway and degradation pathway. |
|---|---|
| ln Vitro |
In vitro, purine and its derivatives have been shown to inhibit the growth of cancer cells by interfering with DNA synthesis. It is used as a substrate to study the purine salvage pathway and as a standard for purine metabolism assays.
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| ln Vivo |
In vivo, purine metabolism is critical for health and disease. Purine analogs (e.g., 6-mercaptopurine, azathioprine) are used as chemotherapeutic agents and immunosuppressants. The unlabeled compound is used to study hyperuricemia and gout by feeding purine-rich diets to animals.
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| Enzyme Assay |
A non-cell assay using purified xanthine oxidase (XO) and purine as a substrate is performed. The conversion of purine to uric acid is monitored spectrophotometrically by measuring the increase in absorbance at 290 nm, which corresponds to the production of uric acid. The reaction is performed in phosphate buffer, pH 7.5, at 25degC.
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| Cell Assay |
Human cancer cell lines (e.g., MCF-7, HeLa) are cultured in the presence of purine or its analogs (1-100 uM). Cell viability is measured by MTT assay. DNA synthesis is assessed by [3H]-thymidine incorporation. Purine levels in cells and culture medium are analyzed by HPLC or LC-MS.
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| Animal Protocol |
In animal studies, purine is typically administered to rodents as part of a high-purine diet (e.g., yeast extract) to induce hyperuricemia and gout-like symptoms. Blood uric acid levels are measured by enzymatic colorimetric assay. This model is used to test the efficacy of xanthine oxidase inhibitors such as allopurinol.
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| ADME/Pharmacokinetics |
Purine is water-soluble and rapidly absorbed from the gut. It is extensively metabolized by the liver, primarily by xanthine oxidase and purine nucleoside phosphorylase. The half-life of purine in the blood is very short (minutes). It is converted to uric acid, which is excreted by the kidneys.
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| Toxicity/Toxicokinetics |
Purine is generally recognized as safe in normal dietary amounts. However, high levels of purine in the diet can lead to hyperuricemia and gout in susceptible individuals due to the production of uric acid. In animal studies, high-purine diets are well tolerated but lead to elevated uric acid levels.
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| Additional Infomation |
7H-purine is the 7H-tautomer of purine. It is a tautomer of 1H-purine, 3H-purine, and 9H-purine. Purines are metabolites present in or produced by Escherichia coli (K12 strain, MG1655 strain). Purines have been reported in humans, ginseng, and cattle, but relevant data are unclear. Purines are also present in or produced by Saccharomyces cerevisiae.
Purine is not a drug itself, but it is the core structure for many important pharmaceuticals, including antiviral drugs (acyclovir), chemotherapeutic agents (6-mercaptopurine, fludarabine), immunosuppressants (azathioprine), and anti-gout medications (allopurinol). It is a fundamental building block in medicinal chemistry. |
| Molecular Formula |
C5H4N4
|
|---|---|
| Molecular Weight |
120.11
|
| Exact Mass |
120.043
|
| CAS # |
120-73-0
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| PubChem CID |
1044
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| Appearance |
Off-white to gray solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
424ºC at 760mmHg
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| Melting Point |
214 °C
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| Flash Point |
225.4ºC
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| Vapour Pressure |
0.0976mmHg at 25°C
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| Index of Refraction |
1.738
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| LogP |
-0.37
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
9
|
| Complexity |
105
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=C2C(=NC=N1)N=CN2
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| InChi Key |
KDCGOANMDULRCW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C5H4N4/c1-4-5(8-2-6-1)9-3-7-4/h1-3H,(H,6,7,8,9)
|
| Chemical Name |
7H-purine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~50 mg/mL (~416.29 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (20.81 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (20.81 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (20.81 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 8.3257 mL | 41.6285 mL | 83.2570 mL | |
| 5 mM | 1.6651 mL | 8.3257 mL | 16.6514 mL | |
| 10 mM | 0.8326 mL | 4.1629 mL | 8.3257 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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