| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
|
||
| Other Sizes |
| Targets |
Human Endogenous Metabolite
Pentane-1,5-diamine targets polyamine metabolism pathways. It is a substrate for diamine oxidase and copper-dependent amine oxidases. It also interacts with cellular polyamine transport systems and can affect ornithine decarboxylase (ODC) activity, the rate-limiting enzyme in polyamine biosynthesis. |
|---|---|
| ln Vitro |
In vitro, Pentane-1,5-diamine has been shown to inhibit ornithine decarboxylase (ODC), affecting spermidine and spermine synthesis. It can be used in bacterial culture studies to investigate polyamine transport and metabolism. It also serves as a substrate for amine oxidase enzymes in biochemical assays.
|
| ln Vivo |
In animal studies, Pentane-1,5-diamine dihydrochloride is used to study polyamine metabolism in vivo. It is administered to rodents via intraperitoneal injection to investigate its effects on cell proliferation, differentiation, and apoptosis. It can also be used to model polyamine dysregulation.
|
| Enzyme Assay |
A non-cell assay involves using Pentane-1,5-diamine as a substrate for diamine oxidase or copper-dependent amine oxidase. The reaction produces hydrogen peroxide and aldehyde products. Activity is measured spectrophotometrically via a coupled assay with HRP and a chromogenic substrate (e.g., o-dianisidine or Amplex Red).
|
| Cell Assay |
For cell studies, Pentane-1,5-diamine dihydrochloride is dissolved in culture medium (1-10 mM, pH adjusted) and added to bacterial or eukaryotic cell cultures. It is used to study polyamine transport in E. coli, to investigate the effects of exogenous diamines on cell proliferation, and as a tool to deplete intracellular polyamine pools.
|
| Animal Protocol |
In animal experiments, Pentane-1,5-diamine dihydrochloride is typically administered to rodents by intraperitoneal injection (50-200 mg/kg) or added to drinking water. Chronic treatment leads to inhibition of ornithine decarboxylase activity and reduction of spermidine levels. Tissues are collected for polyamine analysis by HPLC.
|
| ADME/Pharmacokinetics |
Pentane-1,5-diamine is absorbed from the gut and distributed to various tissues. It is rapidly metabolized by diamine oxidase and polyamine oxidase, with a short half-life in circulation (minutes). Its oral bioavailability is low due to extensive first-pass metabolism in the intestine and liver.
|
| Toxicity/Toxicokinetics |
Pentane-1,5-diamine is toxic in large doses, with an intraperitoneal LD₅0 in mice of approximately 500 mg/kg. High doses can cause neurotoxicity, including convulsions and tremors, due to interference with neurotransmitter systems. The dihydrochloride salt may cause local irritation.
|
| Additional Infomation |
Cadaverine dihydrochloride is a hydrochloride formed by the reaction of cadaverine with 2 mol equivalents of hydrogen chloride. It contains cadaverine (2+).
Pentane-1,5-diamine dihydrochloride is used as a biochemical tool in polyamine research, as a standard for GC-MS and LC-MS analysis of biogenic amines in food and clinical samples, and for studying bacterial metabolism (e.g., cadaverine tolerance in E. coli K12). It is not an approved drug. |
| Molecular Formula |
C5H16CL2N2
|
|---|---|
| Molecular Weight |
175.10
|
| Exact Mass |
174.069
|
| CAS # |
1476-39-7
|
| PubChem CID |
80282
|
| Appearance |
White to off-white solid powder
|
| Density |
1.03 g/mL at 20 °C
|
| Boiling Point |
179ºC at 760 mmHg
|
| Melting Point |
260-262 °C(lit.)
|
| Flash Point |
76.5ºC
|
| Vapour Pressure |
0.961mmHg at 25°C
|
| LogP |
3.078
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
9
|
| Complexity |
25.3
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C(CCN)CCN.Cl
|
| InChi Key |
FGNLEIGUMSBZQP-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C5H14N2.2ClH/c6-4-2-1-3-5-7;;/h1-7H2;2*1H
|
| Chemical Name |
pentane-1,5-diamine;dihydrochloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO :~100 mg/mL (~571.10 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (14.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (14.28 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (14.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.7110 mL | 28.5551 mL | 57.1102 mL | |
| 5 mM | 1.1422 mL | 5.7110 mL | 11.4220 mL | |
| 10 mM | 0.5711 mL | 2.8555 mL | 5.7110 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
