| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
This compound is a metabolite involved in regulating metal ion homeostasis and may influence cellular oxidative stress pathways. It is a reagent used to synthesize uridine phosphorylase inhibitors that increase uridine levels.
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|---|---|
| ln Vitro |
In vitro, 6-Hydroxypyridin-2(1H)-one is used as a reagent to synthesize uridine phosphorylase inhibitors. Uridine is a promising biochemical modulator to reduce 5-fluorouracil (5-FU) toxicity without impairing its antitumor activity.
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| ln Vivo |
In vivo, uridine phosphorylase inhibitors synthesized from this compound increase uridine levels, which can reduce 5-FU toxicity while preserving its antitumor efficacy.
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| Enzyme Assay |
A non-cell assay uses purified uridine phosphorylase enzyme and uridine as a substrate. The enzyme activity is measured in the presence of inhibitors synthesized from this compound by monitoring the production of uracil by HPLC.
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| Cell Assay |
For cell studies, 6-Hydroxypyridin-2(1H)-one or its derivatives are added to culture medium of cancer cells in combination with 5-FU. Cell viability is measured by MTT assay to assess the effect on 5-FU cytotoxicity.
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| Animal Protocol |
In animal studies, uridine phosphorylase inhibitors derived from this compound are administered to rodents along with 5-FU to study their ability to reduce 5-FU-induced gastrointestinal and hematological toxicity.
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| ADME/Pharmacokinetics |
Limited PK data are available. The compound itself is a small, polar molecule with good water solubility. Its derivatives have variable PK properties depending on their structure.
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| Toxicity/Toxicokinetics |
The compound is generally considered safe at research doses. The hydrochloride salt may be irritating.
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| Additional Infomation |
6-Hydroxypyridin-2(1H)-one hydrochloride is used as a chemical reagent and building block for the synthesis of uridine phosphorylase inhibitors with potential applications in cancer chemotherapy to reduce 5-FU toxicity. It is also used in research on neurochemistry, metalloprotein interactions, and metabolic disorders.
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| Molecular Formula |
C5H6CLNO2
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|---|---|
| Molecular Weight |
147.56
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| Exact Mass |
147.008
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| CAS # |
10357-84-3
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| Related CAS # |
626-06-2 (Parent)
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| PubChem CID |
3084068
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| Appearance |
White to off-white solid powder
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| Density |
1.379g/cm3
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| Boiling Point |
387.2ºC at 760mmHg
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| Melting Point |
206-208 °C (dec.)(lit.)
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| Flash Point |
188ºC
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| Vapour Pressure |
1.35E-07mmHg at 25°C
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| LogP |
1.294
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
9
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| Complexity |
169
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC(=O)NC(=C1)O.Cl
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| InChi Key |
HNWWAWKDVFVJRG-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C5H5NO2.ClH/c7-4-2-1-3-5(8)6-4;/h1-3H,(H2,6,7,8);1H
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| Chemical Name |
6-hydroxy-1H-pyridin-2-one;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~100 mg/mL (~677.69 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (16.94 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (14.10 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (14.10 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7769 mL | 33.8845 mL | 67.7690 mL | |
| 5 mM | 1.3554 mL | 6.7769 mL | 13.5538 mL | |
| 10 mM | 0.6777 mL | 3.3885 mL | 6.7769 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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