| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Ethylene glycol-d4 does not have a specific biological target; it is used as an internal standard for the quantification of ethylene glycol in toxicological and forensic analysis; it may also be used as a tracer in metabolic studies.
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|---|---|
| ln Vitro |
In vitro, Ethylene glycol-d4 is used as an internal standard in LC-MS/MS and GC-MS methods for the analysis of ethylene glycol in biological fluids; it is also used in studies of ethylene glycol metabolism to track the formation of toxic metabolites such as glycolic acid and oxalic acid.
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| ln Vivo |
In vivo, Ethylene glycol-d4 is administered to animal models as a tracer to study the toxicokinetics of ethylene glycol; the deuterium label allows precise quantification of ethylene glycol and its metabolites in blood, urine, and tissues.
|
| Enzyme Assay |
Non-cellular assay procedure: Ethylene glycol-d4 is spiked into biological samples (plasma, serum, or urine) as an internal standard; samples are diluted, centrifuged, and analyzed by LC-MS/MS or GC-MS; the deuterated compound co-elutes with unlabeled ethylene glycol but is detected at a different mass-to-charge ratio for accurate quantification.
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| Cell Assay |
Cellular assays are not typically performed with Ethylene glycol-d4 as it is an analytical standard; it may be used in cell culture to study ethylene glycol uptake or metabolism, but it does not induce pharmacological responses.
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| Animal Protocol |
In vivo animal experiments: Ethylene glycol-d4 is administered to rodents via oral gavage or intravenous injection; blood and urine samples are collected at various time points; ethylene glycol and its metabolites (glycolic acid, glyoxylic acid, and oxalic acid) are quantified by LC-MS/MS using the deuterated compound as an internal standard to study toxicokinetics.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties: Ethylene glycol-d4 behaves identically to unlabeled ethylene glycol; it is rapidly absorbed after oral administration, distributed throughout body water, metabolized in the liver by alcohol dehydrogenase and aldehyde dehydrogenase to toxic metabolites, and excreted in urine; the deuterium label does not significantly alter its pharmacokinetics.
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| Toxicity/Toxicokinetics |
Toxicological profile: Ethylene glycol-d4 is a stable isotope-labeled compound with the same toxicological properties as unlabeled ethylene glycol; ethylene glycol is toxic if ingested, causing metabolic acidosis, renal failure, and central nervous system depression; handling requires appropriate safety precautions.
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| References | |
| Additional Infomation |
Other information: Ethylene glycol-d4 has a molecular formula of C₂D₄H₂O₂ and a molecular weight of approximately 66.08; it is used as an internal standard in clinical toxicology for the diagnosis of ethylene glycol poisoning; its role is primarily analytical rather than pharmacological.
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| Molecular Formula |
C2H2D4O2
|
|---|---|
| Molecular Weight |
66.09
|
| Exact Mass |
66.061
|
| CAS # |
2219-51-4
|
| PubChem CID |
2733137
|
| Appearance |
Colorless to light yellow liquid
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| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
197.5±0.0 °C at 760 mmHg
|
| Melting Point |
-13ºC(lit.)
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| Flash Point |
108.2±13.0 °C
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| Vapour Pressure |
0.1±0.8 mmHg at 25°C
|
| Index of Refraction |
1.422
|
| LogP |
-1.69
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
4
|
| Complexity |
6
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
[2H]C([2H])(C([2H])([2H])O)O
|
| InChi Key |
LYCAIKOWRPUZTN-LNLMKGTHSA-N
|
| InChi Code |
InChI=1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2/i1D2,2D2
|
| Chemical Name |
1,1,2,2-tetradeuterioethane-1,2-diol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O :~200 mg/mL (~3026.18 mM)
DMSO :~100 mg/mL (~1513.09 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (37.83 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (37.83 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (37.83 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 15.1309 mL | 75.6544 mL | 151.3088 mL | |
| 5 mM | 3.0262 mL | 15.1309 mL | 30.2618 mL | |
| 10 mM | 1.5131 mL | 7.5654 mL | 15.1309 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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